Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 2605807..2606606 Replicon chromosome
Accession NZ_CP120633
Organism Escherichia coli strain USVAST219

Toxin (Protein)


Gene name yhaV Uniprot ID S1NYM6
Locus tag P6O79_RS13040 Protein ID WP_000347251.1
Coordinates 2606142..2606606 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1PPV5
Locus tag P6O79_RS13035 Protein ID WP_001296435.1
Coordinates 2605807..2606142 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
P6O79_RS13020 (2601592) 2601592..2602362 - 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -
P6O79_RS13025 (2602378) 2602378..2603712 - 1335 WP_277715573.1 galactarate/glucarate/glycerate transporter GarP -
P6O79_RS13030 (2604087) 2604087..2605658 + 1572 WP_001273763.1 galactarate dehydratase -
P6O79_RS13035 (2605807) 2605807..2606142 + 336 WP_001296435.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
P6O79_RS13040 (2606142) 2606142..2606606 + 465 WP_000347251.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
P6O79_RS13045 (2606661) 2606661..2607470 - 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
P6O79_RS13050 (2607719) 2607719..2608999 + 1281 WP_000681950.1 tagatose-bisphosphate aldolase subunit KbaZ -
P6O79_RS13055 (2609022) 2609022..2609495 + 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
P6O79_RS13060 (2609506) 2609506..2610285 + 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
P6O79_RS13065 (2610275) 2610275..2611153 + 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
P6O79_RS13070 (2611171) 2611171..2611605 + 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17777.14 Da        Isoelectric Point: 9.4947

>T275300 WP_000347251.1 NZ_CP120633:2606142-2606606 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12321.92 Da        Isoelectric Point: 4.5392

>AT275300 WP_001296435.1 NZ_CP120633:2605807-2606142 [Escherichia coli]
MPANARSNAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFDIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CJ20

Antitoxin

Source ID Structure
AlphaFold DB S1PPV5

References