Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3864734..3865428 Replicon chromosome
Accession NZ_CP120568
Organism Escherichia coli strain 582

Toxin (Protein)


Gene name yafO Uniprot ID A0A3P5GWS5
Locus tag P5711_RS19095 Protein ID WP_001263488.1
Coordinates 3864734..3865132 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag P5711_RS19100 Protein ID WP_000554758.1
Coordinates 3865135..3865428 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
- (3860322) 3860322..3860402 - 81 NuclAT_13 - -
- (3860322) 3860322..3860402 - 81 NuclAT_13 - -
- (3860322) 3860322..3860402 - 81 NuclAT_13 - -
- (3860322) 3860322..3860402 - 81 NuclAT_13 - -
P5711_RS19070 (3860998) 3860998..3861456 - 459 WP_001291990.1 xanthine phosphoribosyltransferase -
P5711_RS19075 (3861717) 3861717..3863174 + 1458 WP_001292994.1 cytosol nonspecific dipeptidase -
P5711_RS19080 (3863231) 3863231..3863752 - 522 Protein_3733 peptide chain release factor H -
P5711_RS19085 (3863748) 3863748..3863954 - 207 Protein_3734 RtcB family protein -
P5711_RS19090 (3864272) 3864272..3864724 - 453 WP_001059892.1 GNAT family N-acetyltransferase -
P5711_RS19095 (3864734) 3864734..3865132 - 399 WP_001263488.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
P5711_RS19100 (3865135) 3865135..3865428 - 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
P5711_RS19105 (3865480) 3865480..3866535 - 1056 WP_001226164.1 DNA polymerase IV -
P5711_RS19110 (3866606) 3866606..3867391 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
P5711_RS19115 (3867363) 3867363..3869075 + 1713 Protein_3740 flagellar biosynthesis protein FlhA -
P5711_RS19120 (3869299) 3869299..3869796 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15559.90 Da        Isoelectric Point: 6.9798

>T275218 WP_001263488.1 NZ_CP120568:c3865132-3864734 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQEAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT275218 WP_000554758.1 NZ_CP120568:c3865428-3865135 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3P5GWS5

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References