Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 4186450..4187144 Replicon chromosome
Accession NZ_CP120567
Organism Escherichia coli strain A0 34/86

Toxin (Protein)


Gene name yafO Uniprot ID S1PJQ2
Locus tag P5707_RS20375 Protein ID WP_001263500.1
Coordinates 4186450..4186848 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag P5707_RS20380 Protein ID WP_000554758.1
Coordinates 4186851..4187144 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
P5707_RS20350 (4181815) 4181815..4182273 - 459 WP_001291991.1 xanthine phosphoribosyltransferase -
P5707_RS20355 (4182534) 4182534..4183991 + 1458 WP_001292999.1 cytosol nonspecific dipeptidase -
P5707_RS20360 (4184048) 4184048..4184662 - 615 WP_000602123.1 peptide chain release factor H -
P5707_RS20365 (4184659) 4184659..4185798 - 1140 WP_000521561.1 RNA ligase RtcB family protein -
P5707_RS20370 (4185988) 4185988..4186440 - 453 WP_001059897.1 GNAT family N-acetyltransferase -
P5707_RS20375 (4186450) 4186450..4186848 - 399 WP_001263500.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
P5707_RS20380 (4186851) 4186851..4187144 - 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
P5707_RS20385 (4187196) 4187196..4188251 - 1056 WP_001226168.1 DNA polymerase IV -
P5707_RS20390 (4188322) 4188322..4189107 - 786 WP_000207578.1 putative lateral flagellar export/assembly protein LafU -
P5707_RS20395 (4189079) 4189079..4190791 + 1713 Protein_3988 flagellar biosynthesis protein FlhA -
P5707_RS20400 (4190870) 4190870..4191115 + 246 WP_133258463.1 hypothetical protein -
P5707_RS20405 (4191110) 4191110..4191607 - 498 WP_000006241.1 REP-associated tyrosine transposase RayT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15413.85 Da        Isoelectric Point: 8.9511

>T275197 WP_001263500.1 NZ_CP120567:c4186848-4186450 [Escherichia coli]
MRVFKTKLIRLQLTAEELEALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDAAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT275197 WP_000554758.1 NZ_CP120567:c4187144-4186851 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PJQ2

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References