Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HTH_XRE
Location 3910960..3911639 Replicon chromosome
Accession NZ_CP120567
Organism Escherichia coli strain A0 34/86

Toxin (Protein)


Gene name higB Uniprot ID V0VBP6
Locus tag P5707_RS19050 Protein ID WP_000057524.1
Coordinates 3911337..3911639 (-) Length 101 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1QAY3
Locus tag P5707_RS19045 Protein ID WP_000806442.1
Coordinates 3910960..3911301 (-) Length 114 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
P5707_RS19035 (3907204) 3907204..3908136 - 933 WP_000883041.1 glutaminase A -
P5707_RS19040 (3908398) 3908398..3910902 + 2505 WP_021513535.1 copper-exporting P-type ATPase CopA -
P5707_RS19045 (3910960) 3910960..3911301 - 342 WP_000806442.1 HigA family addiction module antitoxin Antitoxin
P5707_RS19050 (3911337) 3911337..3911639 - 303 WP_000057524.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
P5707_RS19055 (3911772) 3911772..3912566 + 795 WP_000365177.1 TraB/GumN family protein -
P5707_RS19060 (3912770) 3912770..3913249 + 480 WP_000186633.1 Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK -
P5707_RS19065 (3913273) 3913273..3914073 + 801 WP_000439798.1 hypothetical protein -
P5707_RS19070 (3914070) 3914070..3914573 + 504 WP_000667000.1 hypothetical protein -
P5707_RS19075 (3914611) 3914611..3916263 - 1653 WP_000771760.1 bifunctional UDP-sugar hydrolase/5'-nucleotidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(9-98)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-100)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11839.47 Da        Isoelectric Point: 10.2237

>T275195 WP_000057524.1 NZ_CP120567:c3911639-3911337 [Escherichia coli]
MAQKKNIRSFRDAWLADFFVHSTPHRKIPAEIHTTLSRKLDIINAATSHWDLRSPPGNRYEELSGKLQEYSSIRVNKQYR
LIFKWVNGKAEELFLDPHNY

Download         Length: 303 bp

Antitoxin


Download         Length: 114 a.a.        Molecular weight: 13171.10 Da        Isoelectric Point: 5.7790

>AT275195 WP_000806442.1 NZ_CP120567:c3911301-3910960 [Escherichia coli]
MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSALINNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLW
EVENNMRTQEELGRIETVAEYLARREERAKKVA

Download         Length: 342 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0VBP6

Antitoxin

Source ID Structure
PDB 2EBY
AlphaFold DB S1QAY3

References