Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2546520..2547073 Replicon chromosome
Accession NZ_CP120563
Organism Escherichia coli strain H22

Toxin (Protein)


Gene name higB Uniprot ID A0A0G9FUJ7
Locus tag P5708_RS12480 Protein ID WP_000605680.1
Coordinates 2546520..2546789 (+) Length 90 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag P5708_RS12485 Protein ID WP_000781370.1
Coordinates 2546789..2547073 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
P5708_RS12455 (2542106) 2542106..2542537 - 432 WP_000152307.1 peroxiredoxin OsmC -
P5708_RS12460 (2542882) 2542882..2543097 + 216 WP_000495766.1 biofilm-dependent modulation protein -
P5708_RS12465 (2543199) 2543199..2543336 + 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
P5708_RS12470 (2543493) 2543493..2545190 + 1698 WP_000433460.1 malate dehydrogenase -
P5708_RS12475 (2545324) 2545324..2546334 + 1011 WP_000642412.1 alcohol dehydrogenase AdhP -
P5708_RS12480 (2546520) 2546520..2546789 + 270 WP_000605680.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
P5708_RS12485 (2546789) 2546789..2547073 + 285 WP_000781370.1 HigA family addiction module antitoxin Antitoxin
P5708_RS12490 (2547302) 2547302..2547955 - 654 WP_000045647.1 formate dehydrogenase-N subunit gamma -
P5708_RS12495 (2547948) 2547948..2548832 - 885 WP_001240584.1 formate dehydrogenase N subunit beta -
P5708_RS12500 (2548845) 2548845..2551892 - 3048 WP_011478186.1 formate dehydrogenase-N subunit alpha -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(1-89)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 90 a.a.        Molecular weight: 10181.73 Da        Isoelectric Point: 6.8582

>T275165 WP_000605680.1 NZ_CP120563:2546520-2546789 [Escherichia coli]
MIMNFRHKGLRDLFLLGKTSGVIPTRLRHRLAVIDAACCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVNGDA
YILDYEDYH

Download         Length: 270 bp

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT275165 WP_000781370.1 NZ_CP120563:2546789-2547073 [Escherichia coli]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0G9FUJ7

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References