Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 4410948..4411489 Replicon chromosome
Accession NZ_CP120557
Organism Escherichia coli strain B771

Toxin (Protein)


Gene name yafQ Uniprot ID A0A140NAP5
Locus tag P5709_RS21435 Protein ID WP_000615981.1
Coordinates 4410948..4411226 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag P5709_RS21440 Protein ID WP_000729704.1
Coordinates 4411229..4411489 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
P5709_RS21420 (4408530) 4408530..4409108 + 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -
P5709_RS21425 (4409314) 4409314..4410081 + 768 WP_000333379.1 class II glutamine amidotransferase -
P5709_RS21430 (4410052) 4410052..4410792 - 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
P5709_RS21435 (4410948) 4410948..4411226 - 279 WP_000615981.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
P5709_RS21440 (4411229) 4411229..4411489 - 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
P5709_RS21445 (4411675) 4411675..4412448 + 774 WP_001353765.1 C40 family peptidase -
P5709_RS21450 (4412624) 4412624..4413121 + 498 WP_000006260.1 REP-associated tyrosine transposase RayT -
P5709_RS21455 (4413337) 4413337..4415049 - 1713 Protein_4193 flagellar biosynthesis protein FlhA -
P5709_RS21460 (4415021) 4415021..4415806 + 786 WP_000207564.1 putative lateral flagellar export/assembly protein LafU -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - gmhA/lpcA 4400642..4436752 36110


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(6-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10860.65 Da        Isoelectric Point: 10.0702

>T275139 WP_000615981.1 NZ_CP120557:c4411226-4410948 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQSSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT275139 WP_000729704.1 NZ_CP120557:c4411489-4411229 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MMG
PDB 4MMJ
PDB 4ML2
PDB 4ML0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References