Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2642547..2643274 Replicon chromosome
Accession NZ_CP120557
Organism Escherichia coli strain B771

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag P5709_RS13065 Protein ID WP_000550189.1
Coordinates 2642960..2643274 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag P5709_RS13060 Protein ID WP_000560255.1
Coordinates 2642547..2642963 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
P5709_RS13050 (2637707) 2637707..2640058 + 2352 WP_000695486.1 alpha-glucosidase -
P5709_RS13055 (2640484) 2640484..2642502 + 2019 WP_000121433.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
P5709_RS13060 (2642547) 2642547..2642963 - 417 WP_000560255.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
P5709_RS13065 (2642960) 2642960..2643274 - 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
P5709_RS13070 (2643558) 2643558..2644694 - 1137 WP_000018695.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
P5709_RS13075 (2644779) 2644779..2645282 + 504 WP_001300832.1 M48 family metallopeptidase -
P5709_RS13080 (2645359) 2645359..2646051 + 693 WP_000942548.1 vancomycin high temperature exclusion protein -
P5709_RS13085 (2646130) 2646130..2647116 + 987 WP_000617698.1 Gfo/Idh/MocA family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Antitoxin HigA

(1-138)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T275136 WP_000550189.1 NZ_CP120557:c2643274-2642960 [Escherichia coli]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 14951.41 Da        Isoelectric Point: 4.5577

>AT275136 WP_000560255.1 NZ_CP120557:c2642963-2642547 [Escherichia coli]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNAPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References