Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3891412..3892106 Replicon chromosome
Accession NZ_CP120549
Organism Escherichia coli strain B1172

Toxin (Protein)


Gene name yafO Uniprot ID A0A366YGZ3
Locus tag P5710_RS18905 Protein ID WP_001518358.1
Coordinates 3891412..3891810 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID L4JHF0
Locus tag P5710_RS18910 Protein ID WP_000554755.1
Coordinates 3891813..3892106 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
P5710_RS18880 (3886777) 3886777..3887235 - 459 WP_001291991.1 xanthine phosphoribosyltransferase -
P5710_RS18885 (3887496) 3887496..3888953 + 1458 WP_001292999.1 cytosol nonspecific dipeptidase -
P5710_RS18890 (3889010) 3889010..3889624 - 615 WP_000602123.1 peptide chain release factor H -
P5710_RS18895 (3889621) 3889621..3890760 - 1140 WP_000521578.1 RNA ligase RtcB family protein -
P5710_RS18900 (3890950) 3890950..3891402 - 453 WP_001059897.1 GNAT family N-acetyltransferase -
P5710_RS18905 (3891412) 3891412..3891810 - 399 WP_001518358.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
P5710_RS18910 (3891813) 3891813..3892106 - 294 WP_000554755.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
P5710_RS18915 (3892158) 3892158..3893213 - 1056 WP_001226170.1 DNA polymerase IV -
P5710_RS18920 (3893284) 3893284..3894069 - 786 WP_000207543.1 putative lateral flagellar export/assembly protein LafU -
P5710_RS18925 (3894041) 3894041..3895753 + 1713 Protein_3710 flagellar biosynthesis protein FlhA -
P5710_RS18930 (3895781) 3895781..3896275 + 495 WP_139174989.1 hypothetical protein -
P5710_RS18935 (3896270) 3896270..3896767 - 498 WP_000006241.1 REP-associated tyrosine transposase RayT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 3841270..3897701 56431


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15448.85 Da        Isoelectric Point: 8.0738

>T275109 WP_001518358.1 NZ_CP120549:c3891810-3891412 [Escherichia coli]
MRVFKTKLIRLQLTAEELEALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMNKIGKMAEAFRMRF

Download         Length: 399 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11235.78 Da        Isoelectric Point: 5.2170

>AT275109 WP_000554755.1 NZ_CP120549:c3892106-3891813 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKLVKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A366YGZ3

Antitoxin

Source ID Structure
AlphaFold DB A0A829G9H5

References