Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 1688463..1689025 Replicon chromosome
Accession NC_017651
Organism Escherichia coli str. clone D i2

Toxin (Protein)


Gene name higB Uniprot ID S1Q1V8
Locus tag I02_RS08615 Protein ID WP_000605675.1
Coordinates 1688747..1689025 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag I02_RS08610 Protein ID WP_000781370.1
Coordinates 1688463..1688747 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I02_RS08595 1683644..1686691 + 3048 WP_011076399.1 formate dehydrogenase-N subunit alpha -
I02_RS08600 1686704..1687588 + 885 WP_001240584.1 formate dehydrogenase N subunit beta -
I02_RS08605 1687581..1688234 + 654 WP_000045646.1 formate dehydrogenase-N subunit gamma -
I02_RS08610 1688463..1688747 - 285 WP_000781370.1 HigA family addiction module antidote protein Antitoxin
I02_RS08615 1688747..1689025 - 279 WP_000605675.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
I02_RS08620 1689211..1690221 - 1011 WP_000642412.1 alcohol dehydrogenase AdhP -
I02_RS08625 1690355..1692052 - 1698 WP_000433460.1 malate dehydrogenase -
I02_RS08630 1692209..1692346 - 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
I02_RS08635 1692448..1692663 - 216 WP_000495766.1 biofilm-dependent modulation protein -
I02_RS08640 1693008..1693439 + 432 WP_000152307.1 peroxiredoxin OsmC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10537.16 Da        Isoelectric Point: 7.3206

>T27510 WP_000605675.1 NC_017651:c1689025-1688747 [Escherichia coli str. 'clone D i2']
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAACCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

>T27510 NC_017651:c1689025-1688747 [Escherichia coli str. 'clone D i2']
ATGATAATGAATTTCAGACACAAGGGATTGCGTGACTTGTTTCTTCTCGGCAAAACCTCCGGCGTTATACCGACGCAAGT
CAAACGATTACGCCACCGACTCGCTGTGATTGATGCAGCATGCTGTCTTGCTGATATCGATATGCCCGGTTACCGACTAC
ATCCGTTAAGCGGCGATCGCGATGGAATTTGGGCGATATCTGTCTCGGGTAACTGGCGAATCACATTTGAATTCGTCAAT
GGCGATGCATACATACTGGATTACGAGGACTATCACTGA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT27510 WP_000781370.1 NC_017651:c1688747-1688463 [Escherichia coli str. 'clone D i2']
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp

>AT27510 NC_017651:c1688747-1688463 [Escherichia coli str. 'clone D i2']
ATGAAAATGGCCAATCATCCCCGCCCGGGGGACATTATTCAGGAATCACTGGACGAACTTAATGTCAGCCTGCGCGAGTT
TGCCAGAGCAATGGAAATTGCGCCCTCTACGGCAAGTCGATTGCTGACCGGAAAAGCAGCTTTGACGCCAGAAATGGCAA
TAAAACTGTCTGTGGTGATCGGCAGTTCGCCGCAAATGTGGCTGAATCTGCAAAATGCCTGGAGTCTGGCAGAGGCAGAA
AAAACAGTGGATGTGTCGCGACTGCGCCGTCTGGTTACGCAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1Q1V8

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References