Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 253673..254214 Replicon chromosome
Accession NC_017646
Organism Escherichia coli O7:K1 str. CE10

Toxin (Protein)


Gene name yafQ Uniprot ID D8A3N8
Locus tag CE10_RS01195 Protein ID WP_000615978.1
Coordinates 253673..253951 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID Q32JN2
Locus tag CE10_RS01200 Protein ID WP_000729709.1
Coordinates 253954..254214 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CE10_RS01180 251356..251934 + 579 WP_001570958.1 D-sedoheptulose 7-phosphate isomerase -
CE10_RS01185 252039..252806 + 768 WP_000333380.1 class II glutamine amidotransferase -
CE10_RS01190 252777..253517 - 741 WP_001225679.1 murein L,D-transpeptidase -
CE10_RS01195 253673..253951 - 279 WP_000615978.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
CE10_RS01200 253954..254214 - 261 WP_000729709.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
CE10_RS01205 254400..255173 + 774 WP_015674584.1 C40 family peptidase -
CE10_RS01210 255349..255846 + 498 WP_000006244.1 transposase -
CE10_RS01215 255963..257675 - 1713 Protein_230 flagellar biosynthesis protein FlhA -
CE10_RS01220 257647..258432 + 786 WP_000207579.1 putative lateral flagellar export/assembly protein LafU -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(5-92)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10846.66 Da        Isoelectric Point: 10.0702

>T27474 WP_000615978.1 NC_017646:c253951-253673 [Escherichia coli O7:K1 str. CE10]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNTLLLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T27474 NC_017646:c253951-253673 [Escherichia coli O7:K1 str. CE10]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACTGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9333.73 Da        Isoelectric Point: 5.8129

>AT27474 WP_000729709.1 NC_017646:c254214-253954 [Escherichia coli O7:K1 str. CE10]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVGVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT27474 NC_017646:c254214-253954 [Escherichia coli O7:K1 str. CE10]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAAGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACTATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGGTGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G928

Antitoxin

Source ID Structure
AlphaFold DB A0A829FY17

References