Detailed information of TA system TA027472

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 14747..15404
• Replicon: plasmid pUMNK88
• Accession: NC_017645
• Organism: Escherichia coli UMNK88

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U3PDC3
• Locus tag: UMNK88_RS28495
• Protein ID: WP_000270043.1
• Coordinates: 15054..15404 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: UMNK88_RS28490
• Protein ID: WP_000124640.1
• Coordinates: 14747..15049 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
UMNK88_RS28440	10372..10800	+	429	WP_000591074.1	hypothetical protein	-
UMNK88_RS28445	10857..11216	+	360	WP_000422768.1	hypothetical protein	-
UMNK88_RS28450	11216..11662	+	447	WP_000919345.1	Fe3+-siderophore ABC transporter permease	-
UMNK88_RS28455	11659..12177	+	519	WP_000210756.1	nitrite reductase	-
UMNK88_RS28460	12177..12407	+	231	WP_000972663.1	hypothetical protein	-
UMNK88_RS28465	12394..13251	+	858	WP_001167032.1	hypothetical protein	-
UMNK88_RS28470	13277..13465	+	189	WP_001270411.1	hypothetical protein	-
UMNK88_RS28475	13482..14009	+	528	WP_001236377.1	thermonuclease family protein	-
UMNK88_RS28480	14067..14339	+	273	WP_001043047.1	HU family DNA-binding protein	-
UMNK88_RS28485	14427..14720	+	294	WP_001239997.1	chromosome segregation protein ParM	-
UMNK88_RS28490	14747..15049	-	303	WP_000124640.1	XRE family transcriptional regulator	Antitoxin
UMNK88_RS28495	15054..15404	-	351	WP_000270043.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
UMNK88_RS28500	15567..16115	+	549	WP_001061574.1	transcriptional regulator	-
UMNK88_RS32945	16456..16650	+	195	WP_000343597.1	hypothetical protein	-
UMNK88_RS32950	16661..17032	+	372	WP_000516916.1	hypothetical protein	-
UMNK88_RS28520	17025..17495	+	471	WP_001281821.1	hypothetical protein	-
UMNK88_RS28525	17510..17845	-	336	WP_000683476.1	hypothetical protein	-
UMNK88_RS28530	17942..18430	+	489	WP_001273096.1	DUF1643 domain-containing protein	-
UMNK88_RS28535	18433..18930	+	498	WP_000062185.1	hypothetical protein	-
UMNK88_RS28540	19145..19849	-	705	WP_024250745.1	IS6-like element IS26 family transposase	-
UMNK88_RS28545	19914..20093	-	180	Protein_33	helix-turn-helix transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	floR / tet(A) / aph(6)-Id / aph(3'')-Ib / sul2 / blaCMY-2 / ant(3'')-Ia / cmlA1 / aadA2 / qacE / sul1	-	1..160573	160573

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-79)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13328.21 Da
• Isoelectric Point: 5.2421

>T27472 WP_000270043.1 NC_017645:c15404-15054 [Escherichia coli UMNK88]
MWVIETTDTFDEWFDALDDTDRANVLASMMVLRDRGPMLSRPYADTVNGSSYSNMKELRVQSKGDPIRAFFAFDPKRKGI
LLCAGNKTGDEKRFYEVMIPIADREFAAHLDKLKKE

Nucleotide

• Length: 351 bp

>T27472 NC_017645:c15404-15054 [Escherichia coli UMNK88]
ATGTGGGTCATCGAGACAACCGACACCTTTGATGAGTGGTTCGATGCTCTGGATGATACCGATAGAGCAAACGTGCTGGC
TTCGATGATGGTGCTGCGAGATAGAGGCCCCATGCTGTCGAGGCCATACGCGGATACTGTTAACGGTTCATCCTACAGCA
ACATGAAAGAGCTTCGGGTCCAAAGCAAAGGAGATCCTATCAGAGCGTTCTTTGCGTTCGATCCAAAGCGTAAGGGGATT
CTTCTCTGCGCCGGTAACAAGACCGGGGACGAGAAAAGGTTTTATGAAGTAATGATCCCAATTGCAGACCGTGAGTTTGC
GGCGCACTTGGATAAATTGAAGAAGGAGTGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11006.73 Da
• Isoelectric Point: 7.2036

>AT27472 WP_000124640.1 NC_017645:c15049-14747 [Escherichia coli UMNK88]
MARTLDQMLATEKPEVVAKAQKAATEMLLNIHLAELRDRMNLTQGEIAASLGVRQPTVSEMEKPGRDLKLSSIKRYVEAS
GGKLRLDVELPDGTHYGFAV

Nucleotide

• Length: 303 bp

>AT27472 NC_017645:c15049-14747 [Escherichia coli UMNK88]
ATGGCAAGAACTCTTGACCAAATGCTGGCAACAGAAAAGCCTGAGGTTGTTGCCAAAGCACAGAAAGCGGCCACTGAGAT
GTTACTGAACATCCACTTGGCAGAGCTTCGTGATCGCATGAACCTTACCCAGGGGGAAATTGCTGCATCTCTGGGTGTGA
GACAGCCGACAGTCTCCGAGATGGAAAAACCTGGACGAGATCTCAAGCTGTCGTCCATTAAGCGGTACGTTGAGGCCTCC
GGTGGCAAGCTACGCCTCGATGTTGAACTTCCGGACGGCACCCACTACGGCTTTGCCGTTTAA

Structures

Toxin

• AlphaFold DB: A0A7G8AFB9