Detailed information of TA system TA027467

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 42102..42366
• Replicon: plasmid pUMNK88_91
• Accession: NC_017642
• Organism: Escherichia coli UMNK88

Toxin (Protein)

• Gene name: pndA
• Uniprot ID: E6BRV3
• Locus tag: UMNK88_RS27250
• Protein ID: WP_001303307.1
• Coordinates: 42214..42366 (+)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	pndB
Locus tag	-
Coordinates	42102..42164 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
UMNK88_RS27235	37341..39632	-	2292	WP_001289269.1	TrbC	-
UMNK88_RS27240	39625..40695	-	1071	WP_000151576.1	IncI1-type conjugal transfer protein TrbB	-
UMNK88_RS27245	40714..41922	-	1209	WP_000121273.1	IncI1-type conjugal transfer protein TrbA	-
-	42102..42164	-	63	NuclAT_0	-	Antitoxin
-	42102..42164	-	63	NuclAT_0	-	Antitoxin
-	42102..42164	-	63	NuclAT_0	-	Antitoxin
-	42102..42164	-	63	NuclAT_0	-	Antitoxin
UMNK88_RS27250	42214..42366	+	153	WP_001303307.1	Hok/Gef family protein	Toxin
UMNK88_RS27255	42438..42689	-	252	WP_001291965.1	hypothetical protein	-
-	43076..43127	-	52	NuclAT_1	-	-
-	43076..43127	-	52	NuclAT_1	-	-
-	43076..43127	-	52	NuclAT_1	-	-
-	43076..43127	-	52	NuclAT_1	-	-
UMNK88_RS27260	43422..44072	+	651	WP_000993956.1	IS66 family insertion sequence hypothetical protein	-
UMNK88_RS27265	44072..44419	+	348	WP_000624600.1	IS66 family insertion sequence element accessory protein TnpB	-
UMNK88_RS27270	44439..46010	+	1572	WP_000381458.1	IS66-like element IS679 family transposase	-
UMNK88_RS34515	46326..46502	-	177	WP_001054900.1	hypothetical protein	-
UMNK88_RS27285	46894..47103	+	210	WP_000062603.1	HEAT repeat domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..90868	90868

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-46)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5817.21 Da
• Isoelectric Point: 8.7948

>T27467 WP_001303307.1 NC_017642:42214-42366 [Escherichia coli UMNK88]
MPQRTFLMMLIVICVTILCFVWMVRDSLCVLRLQQGNTVLVATLAYEVKR

Nucleotide

• Length: 153 bp

>T27467 NC_017642:42214-42366 [Escherichia coli UMNK88]
ATGCCACAGCGAACGTTTTTAATGATGTTAATCGTCATCTGTGTGACGATTCTGTGTTTTGTCTGGATGGTGAGGGATTC
GCTTTGCGTACTCCGGCTCCAGCAGGGAAACACAGTGCTTGTGGCAACGTTAGCCTACGAAGTTAAACGTTAA

Antitoxin

Nucleotide

• Length: 63 bp

>AT27467 NC_017642:c42164-42102 [Escherichia coli UMNK88]
TCGAAGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Structures

Toxin

• AlphaFold DB: A0A634Q5L1

Antitoxin