Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2443535..2444037 Replicon chromosome
Accession NC_017641
Organism Escherichia coli UMNK88

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag UMNK88_RS12745 Protein ID WP_000767829.1
Coordinates 2443535..2443789 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag UMNK88_RS12750 Protein ID WP_001259255.1
Coordinates 2443786..2444037 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
UMNK88_RS12720 2438550..2438777 - 228 WP_000985273.1 sulfurtransferase TusA family protein -
UMNK88_RS12725 2438791..2439849 - 1059 WP_000492311.1 transport protein YeeE -
UMNK88_RS12730 2440028..2441386 - 1359 WP_024250698.1 putrescine/proton symporter PlaP -
UMNK88_RS33220 2441376..2441438 - 63 WP_010723108.1 membrane protein YoeI -
UMNK88_RS12735 2441653..2442582 - 930 WP_000803351.1 LysR family transcriptional regulator -
UMNK88_RS12740 2442628..2443452 - 825 WP_000754747.1 SDR family oxidoreductase -
UMNK88_RS12745 2443535..2443789 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
UMNK88_RS12750 2443786..2444037 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
UMNK88_RS30670 2444320..2444370 + 51 WP_001364200.1 his operon leader peptide -
UMNK88_RS12755 2444516..2445415 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
UMNK88_RS12760 2445421..2446725 + 1305 WP_000009594.1 histidinol dehydrogenase -
UMNK88_RS12765 2446722..2447792 + 1071 WP_000108980.1 histidinol-phosphate transaminase -
UMNK88_RS12770 2447792..2448859 + 1068 WP_000080095.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T27458 WP_000767829.1 NC_017641:c2443789-2443535 [Escherichia coli UMNK88]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T27458 NC_017641:c2443789-2443535 [Escherichia coli UMNK88]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT27458 WP_001259255.1 NC_017641:c2444037-2443786 [Escherichia coli UMNK88]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT27458 NC_017641:c2444037-2443786 [Escherichia coli UMNK88]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References