Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-VapI
Location 4422974..4423706 Replicon chromosome
Accession NZ_CP119869
Organism Methylosinus trichosporium OB3b

Toxin (Protein)


Gene name higB Uniprot ID A0A1A6FRG6
Locus tag P3C36_RS20905 Protein ID WP_003615036.1
Coordinates 4422974..4423252 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A1A6FTV8
Locus tag P3C36_RS20910 Protein ID WP_003615038.1
Coordinates 4423380..4423706 (+) Length 109 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
P3C36_RS20885 4418549..4419724 + 1176 WP_024749364.1 IS4 family transposase -
P3C36_RS20890 4419774..4421078 - 1305 WP_003615029.1 folylpolyglutamate synthase/dihydrofolate synthase family protein -
P3C36_RS20895 4421098..4421958 - 861 WP_003615033.1 acetyl-CoA carboxylase, carboxyltransferase subunit beta -
P3C36_RS20900 4421955..4422857 - 903 WP_003615034.1 tryptophan synthase subunit alpha -
P3C36_RS20905 4422974..4423252 + 279 WP_003615036.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
P3C36_RS20910 4423380..4423706 + 327 WP_003615038.1 HigA family addiction module antitoxin Antitoxin
P3C36_RS20915 4423715..4424605 - 891 WP_003615039.1 LysR substrate-binding domain-containing protein -
P3C36_RS20920 4424716..4425168 + 453 WP_003615041.1 cupin domain-containing protein -
P3C36_RS20925 4425177..4426076 + 900 WP_003615043.1 dihydrodipicolinate synthase family protein -
P3C36_RS20930 4426191..4426931 + 741 WP_003615045.1 sulfite exporter TauE/SafE family protein -
P3C36_RS20935 4427045..4427257 + 213 WP_155931257.1 hypothetical protein -
P3C36_RS20940 4427305..4427955 + 651 WP_003615048.1 Fic family protein -
P3C36_RS20945 4428025..4428381 + 357 WP_003615049.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(12-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(20-95)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10329.69 Da        Isoelectric Point: 7.2044

>T274483 WP_003615036.1 NZ_CP119869:4422974-4423252 [Methylosinus trichosporium OB3b]
MIRSVKGTVTRQFVETGKSKFSGLDEALARQRLAELDSAASLDDLPPLRSVGLHRLSGDRKGQWAIKVNGPWRIAFRFED
GDAFEVEIVDYH

Download         Length: 279 bp

Antitoxin


Download         Length: 109 a.a.        Molecular weight: 11696.42 Da        Isoelectric Point: 10.2107

>AT274483 WP_003615038.1 NZ_CP119869:4423380-4423706 [Methylosinus trichosporium OB3b]
MTTKSADIFEAPPGGFRFGPIHPGRTLAAELKARGISAHALALSLRVPANRISEIVAGKRGVTAETALRLARYLGTSAAF
WMNLQSKYDLEIAERDFGERIRAEVEAA

Download         Length: 327 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1A6FRG6

Antitoxin

Source ID Structure
AlphaFold DB A0A1A6FTV8

References