Detailed information of TA system TA027437

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 284851..285545
• Replicon: chromosome
• Accession: NC_017641
• Organism: Escherichia coli UMNK88

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: A0A3A6T3I6
• Locus tag: UMNK88_RS01315
• Protein ID: WP_001263490.1
• Coordinates: 285147..285545 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: UMNK88_RS01310
• Protein ID: WP_000554757.1
• Coordinates: 284851..285144 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
UMNK88_RS01290	280483..280980	+	498	WP_000006255.1	REP-associated tyrosine transposase RayT	-
UMNK88_RS01295	281204..282916	-	1713	Protein_243	flagellar biosynthesis protein FlhA	-
UMNK88_RS01300	282888..283673	+	786	WP_000207587.1	putative lateral flagellar export/assembly protein LafU	-
UMNK88_RS01305	283744..284799	+	1056	WP_001226164.1	DNA polymerase IV	-
UMNK88_RS01310	284851..285144	+	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
UMNK88_RS01315	285147..285545	+	399	WP_001263490.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
UMNK88_RS01320	285555..286007	+	453	WP_001059847.1	GNAT family N-acetyltransferase	-
UMNK88_RS01325	286253..286456	+	204	Protein_249	RNA ligase RtcB family protein	-
UMNK88_RS01330	286455..286976	+	522	Protein_250	peptide chain release factor H	-
UMNK88_RS01335	287033..288490	-	1458	WP_001293003.1	cytosol nonspecific dipeptidase	-
UMNK88_RS01340	288751..289209	+	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
UMNK88_RS01345	289301..290545	+	1245	WP_000189541.1	esterase FrsA	-
-	289805..289885	+	81	NuclAT_8	-	-
-	289805..289885	+	81	NuclAT_8	-	-
-	289805..289885	+	81	NuclAT_8	-	-
-	289805..289885	+	81	NuclAT_8	-	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15486.86 Da
• Isoelectric Point: 8.0949

>T27437 WP_001263490.1 NC_017641:285147-285545 [Escherichia coli UMNK88]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLLYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T27437 NC_017641:285147-285545 [Escherichia coli UMNK88]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGCTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACAGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT27437 WP_000554757.1 NC_017641:284851-285144 [Escherichia coli UMNK88]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT27437 NC_017641:284851-285144 [Escherichia coli UMNK88]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATTACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: A0A3A6T3I6

Antitoxin

• AlphaFold DB: S1FJN6