Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 1163257..1163984 Replicon chromosome
Accession NZ_CP119740
Organism Escherichia coli strain E94

Toxin (Protein)


Gene name higB Uniprot ID J7Q991
Locus tag P1J23_RS05815 Protein ID WP_000547564.1
Coordinates 1163257..1163568 (+) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag P1J23_RS05820 Protein ID WP_000126294.1
Coordinates 1163565..1163984 (+) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
P1J23_RS05785 (1158399) 1158399..1160108 + 1710 WP_001288134.1 formate hydrogenlyase subunit HycE -
P1J23_RS05790 (1160118) 1160118..1160660 + 543 WP_000493792.1 formate hydrogenlyase subunit HycF -
P1J23_RS05795 (1160660) 1160660..1161427 + 768 WP_000067399.1 formate hydrogenlyase subunit HycG -
P1J23_RS05800 (1161424) 1161424..1161834 + 411 WP_001291921.1 formate hydrogenlyase assembly protein HycH -
P1J23_RS05805 (1161827) 1161827..1162297 + 471 WP_000132961.1 hydrogenase maturation peptidase HycI -
P1J23_RS05810 (1162322) 1162322..1163083 + 762 WP_001026446.1 hypothetical protein -
P1J23_RS05815 (1163257) 1163257..1163568 + 312 WP_000547564.1 type II toxin-antitoxin system HigB family toxin Toxin
P1J23_RS05820 (1163565) 1163565..1163984 + 420 WP_000126294.1 helix-turn-helix domain-containing protein Antitoxin
P1J23_RS05825 (1164098) 1164098..1165522 - 1425 WP_000110363.1 6-phospho-beta-glucosidase AscB -
P1J23_RS05830 (1165531) 1165531..1166988 - 1458 WP_001107861.1 PTS cellobiose/arbutin/salicin transporter subunit IIBC -
P1J23_RS05835 (1167248) 1167248..1168258 + 1011 WP_001392554.1 DNA-binding transcriptional regulator AscG -
P1J23_RS05840 (1168407) 1168407..1168934 + 528 WP_276170984.1 electron transport protein HydN -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(22-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(85-138)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12426.13 Da        Isoelectric Point: 9.7248

>T274367 WP_000547564.1 NZ_CP119740:1163257-1163568 [Escherichia coli]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFRYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Download         Length: 312 bp

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15416.42 Da        Isoelectric Point: 4.4596

>AT274367 WP_000126294.1 NZ_CP119740:1163565-1163984 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Download         Length: 420 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A0FJG5

Antitoxin

Source ID Structure

References