Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2912584..2913311 Replicon chromosome
Accession NZ_CP119734
Organism Escherichia coli strain E92

Toxin (Protein)


Gene name higB Uniprot ID J7Q991
Locus tag P1J22_RS14740 Protein ID WP_000547564.1
Coordinates 2913000..2913311 (-) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag P1J22_RS14735 Protein ID WP_000126294.1
Coordinates 2912584..2913003 (-) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
P1J22_RS14715 (2907634) 2907634..2908161 - 528 WP_276170984.1 electron transport protein HydN -
P1J22_RS14720 (2908310) 2908310..2909320 - 1011 WP_001392554.1 DNA-binding transcriptional regulator AscG -
P1J22_RS14725 (2909580) 2909580..2911037 + 1458 WP_001107861.1 PTS cellobiose/arbutin/salicin transporter subunit IIBC -
P1J22_RS14730 (2911046) 2911046..2912470 + 1425 WP_000110363.1 6-phospho-beta-glucosidase AscB -
P1J22_RS14735 (2912584) 2912584..2913003 - 420 WP_000126294.1 helix-turn-helix domain-containing protein Antitoxin
P1J22_RS14740 (2913000) 2913000..2913311 - 312 WP_000547564.1 type II toxin-antitoxin system HigB family toxin Toxin
P1J22_RS14745 (2913485) 2913485..2914246 - 762 WP_001026446.1 hypothetical protein -
P1J22_RS14750 (2914271) 2914271..2914741 - 471 WP_000132961.1 hydrogenase maturation peptidase HycI -
P1J22_RS14755 (2914734) 2914734..2915144 - 411 WP_001291921.1 formate hydrogenlyase assembly protein HycH -
P1J22_RS14760 (2915141) 2915141..2915908 - 768 WP_000067399.1 formate hydrogenlyase subunit HycG -
P1J22_RS14765 (2915908) 2915908..2916450 - 543 WP_000493792.1 formate hydrogenlyase subunit HycF -
P1J22_RS14770 (2916460) 2916460..2918169 - 1710 WP_001288134.1 formate hydrogenlyase subunit HycE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(22-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(85-138)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12426.13 Da        Isoelectric Point: 9.7248

>T274344 WP_000547564.1 NZ_CP119734:c2913311-2913000 [Escherichia coli]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFRYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Download         Length: 312 bp

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15416.42 Da        Isoelectric Point: 4.4596

>AT274344 WP_000126294.1 NZ_CP119734:c2913003-2912584 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Download         Length: 420 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A0FJG5

Antitoxin

Source ID Structure

References