Detailed information of TA system TA027432

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 60316..60742
• Replicon: plasmid pUMNK88_Ent
• Accession: NC_017640
• Organism: Escherichia coli UMNK88

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: UMNK88_RS27865
• Protein ID: WP_001312861.1
• Coordinates: 60316..60474 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	60518..60742 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
UMNK88_RS27830	55366..55593	-	228	WP_001254386.1	conjugal transfer relaxosome protein TraY	-
UMNK88_RS27835	55687..56373	-	687	WP_000332484.1	PAS domain-containing protein	-
UMNK88_RS27840	56564..56946	-	383	Protein_62	relaxosome protein TraM	-
UMNK88_RS27845	57282..57872	+	591	WP_000252680.1	transglycosylase SLT domain-containing protein	-
UMNK88_RS27850	58169..58990	-	822	WP_001234469.1	DUF945 domain-containing protein	-
UMNK88_RS27855	59110..59397	-	288	WP_000107537.1	hypothetical protein	-
UMNK88_RS27865	60316..60474	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	60518..60742	-	225	NuclAT_0	-	Antitoxin
-	60518..60742	-	225	NuclAT_0	-	Antitoxin
-	60518..60742	-	225	NuclAT_0	-	Antitoxin
-	60518..60742	-	225	NuclAT_0	-	Antitoxin
UMNK88_RS27875	60754..61473	-	720	WP_001276278.1	plasmid SOS inhibition protein A	-
UMNK88_RS31890	61470..61903	-	434	Protein_68	conjugation system SOS inhibitor PsiB	-
UMNK88_RS27885	61958..63916	-	1959	WP_000117205.1	ParB/RepB/Spo0J family partition protein	-
UMNK88_RS27890	63980..64213	-	234	WP_000005985.1	DUF905 family protein	-
UMNK88_RS27895	64275..64814	-	540	WP_000290811.1	single-stranded DNA-binding protein	-
UMNK88_RS34525	64840..65046	-	207	WP_000275848.1	hypothetical protein	-
UMNK88_RS34560	65116..65542	+	427	Protein_73	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	eltA / eltB	1..81475	81475

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T27432 WP_001312861.1 NC_017640:c60474-60316 [Escherichia coli UMNK88]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T27432 NC_017640:c60474-60316 [Escherichia coli UMNK88]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 225 bp

>AT27432 NC_017640:c60742-60518 [Escherichia coli UMNK88]
TCATACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGGATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin