Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 607211..608010 Replicon chromosome
Accession NZ_CP119577
Organism Escherichia coli strain C012_chr

Toxin (Protein)


Gene name yhaV Uniprot ID F4VJD3
Locus tag P1J04_RS02990 Protein ID WP_000347266.1
Coordinates 607211..607675 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag P1J04_RS02995 Protein ID WP_001307405.1
Coordinates 607675..608010 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
P1J04_RS02960 (602212) 602212..602646 - 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
P1J04_RS02965 (602664) 602664..603542 - 879 WP_001298758.1 PTS N-acetylgalactosamine transporter subunit IID -
P1J04_RS02970 (603532) 603532..604311 - 780 WP_112039668.1 PTS N-acetylgalactosamine transporter subunit IIC -
P1J04_RS02975 (604322) 604322..604795 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
P1J04_RS02980 (604818) 604818..606098 - 1281 WP_000681921.1 tagatose-bisphosphate aldolase subunit KbaZ -
P1J04_RS02985 (606347) 606347..607156 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
P1J04_RS02990 (607211) 607211..607675 - 465 WP_000347266.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
P1J04_RS02995 (607675) 607675..608010 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
P1J04_RS03000 (608159) 608159..609730 - 1572 WP_001273741.1 galactarate dehydratase -
P1J04_RS03005 (610105) 610105..611439 + 1335 WP_112039669.1 galactarate/glucarate/glycerate transporter GarP -
P1J04_RS03010 (611455) 611455..612225 + 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 607211..619945 12734


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17841.18 Da        Isoelectric Point: 9.4947

>T274225 WP_000347266.1 NZ_CP119577:c607675-607211 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRFFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT274225 WP_001307405.1 NZ_CP119577:c608010-607675 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A836NGD2

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References