Detailed information of TA system TA273880

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 4581019..4581689
• Replicon: chromosome
• Accession: NZ_CP119165
• Organism: Citrobacter freundii strain 2075

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A6N6JXY6
• Locus tag: P0S03_RS22370
• Protein ID: WP_046671374.1
• Coordinates: 4581387..4581689 (-)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: P0S03_RS22365
• Protein ID: WP_046671375.1
• Coordinates: 4581019..4581360 (-)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
P0S03_RS22335 (4576560)	4576560..4577318	+	759	WP_003844769.1	phosphonate C-P lyase system protein PhnK	-
P0S03_RS22340 (4577408)	4577408..4578088	+	681	WP_003841104.1	phosphonate C-P lyase system protein PhnL	-
P0S03_RS22345 (4578085)	4578085..4579221	+	1137	WP_172750600.1	alpha-D-ribose 1-methylphosphonate 5-triphosphate diphosphatase	-
P0S03_RS22350 (4579224)	4579224..4579778	+	555	WP_003844764.1	ribose 1,5-bisphosphokinase	-
P0S03_RS22355 (4579765)	4579765..4580199	+	435	WP_172750601.1	aminoalkylphosphonate N-acetyltransferase	-
P0S03_RS22360 (4580208)	4580208..4580966	+	759	WP_172750602.1	phosphonate metabolism protein PhnP	-
P0S03_RS22365 (4581019)	4581019..4581360	-	342	WP_046671375.1	HigA family addiction module antitoxin	Antitoxin
P0S03_RS22370 (4581387)	4581387..4581689	-	303	WP_046671374.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
P0S03_RS22375 (4581845)	4581845..4582174	-	330	WP_003031807.1	DDRRRQL repeat protein YjdP	-
P0S03_RS22380 (4582244)	4582244..4584508	-	2265	WP_115454677.1	hybrid sensor histidine kinase/response regulator	-
P0S03_RS22385 (4584623)	4584623..4586146	+	1524	WP_003844755.1	sugar ABC transporter ATP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (9-98)

Antitoxin

• Cro/C1-type helix-turn-helix domain (23-70)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11819.43 Da
• Isoelectric Point: 9.9581

>T273880 WP_046671374.1 NZ_CP119165:c4581689-4581387 [Citrobacter freundii]
MSKSLNIRSFRDTWLEDFFERATSHRKIPADIHTALARKLDIINAAVSHRDLRSPPGNRYEELTGKLQEYSSIRVNKQYR
LIFKWVNGKAEDVYLDPHIY

Nucleotide

• Length: 303 bp

>T273880 NZ_CP119165:c4581689-4581387 [Citrobacter freundii]
ATGTCTAAATCATTAAATATCAGGAGCTTTCGGGATACATGGCTTGAAGATTTTTTTGAGCGGGCGACATCGCACAGAAA
AATACCAGCGGATATTCATACTGCATTAGCCAGAAAGCTGGATATCATTAATGCGGCTGTTTCGCATCGGGATTTGCGCT
CGCCACCCGGTAACAGATATGAAGAGTTAACCGGAAAATTACAGGAATATTCTTCAATCAGAGTAAACAAGCAGTACCGA
TTAATATTTAAATGGGTTAACGGTAAGGCCGAAGATGTGTACTTAGATCCACACATCTACTAG

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 13017.84 Da
• Isoelectric Point: 7.2040

>AT273880 WP_046671375.1 NZ_CP119165:c4581360-4581019 [Citrobacter freundii]
MKQATRKPTTVGDILQYEYLEPLGLKINDLAEILHVHRNTVSALVNNNRKLTTDMAFRLAKAFDTSVDFWRNLQAAVDLW
EVENDMRAQEELNRIVSVKDFIAQRNSESKKVA

Nucleotide

• Length: 342 bp

>AT273880 NZ_CP119165:c4581360-4581019 [Citrobacter freundii]
ATGAAACAGGCAACCAGAAAACCAACGACTGTGGGTGATATCCTGCAATATGAATACCTGGAGCCGCTCGGACTGAAAAT
TAACGATCTGGCTGAAATACTGCATGTTCATCGCAATACGGTGAGCGCACTGGTCAACAATAATCGCAAATTAACAACGG
ATATGGCGTTTCGTTTGGCCAAAGCGTTCGATACCTCCGTTGATTTCTGGCGTAACTTACAGGCGGCGGTTGACCTGTGG
GAAGTTGAAAATGACATGCGGGCGCAAGAAGAACTGAACCGCATTGTTTCGGTTAAAGATTTTATCGCTCAGCGAAATAG
CGAGTCTAAAAAAGTCGCCTGA

Structures

Toxin

• AlphaFold DB: A0A6N6JXY6