Detailed information of TA system TA027382

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4303804..4304406
• Replicon: chromosome
• Accession: NC_017635
• Organism: Escherichia coli W

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: ECW_RS21460
• Protein ID: WP_000897305.1
• Coordinates: 4303804..4304115 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: ECW_RS21465
• Protein ID: WP_000356397.1
• Coordinates: 4304116..4304406 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECW_RS21430	4298834..4299619	+	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-
ECW_RS21435	4299718..4300317	+	600	WP_001315111.1	glucose-1-phosphatase	-
ECW_RS21440	4300311..4301183	+	873	WP_000920762.1	virulence factor BrkB family protein	-
ECW_RS21445	4301180..4301617	+	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
ECW_RS21450	4301662..4302603	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
ECW_RS21455	4302667..4303575	-	909	WP_001162704.1	alpha/beta hydrolase	-
ECW_RS21460	4303804..4304115	+	312	WP_000897305.1	hypothetical protein	Toxin
ECW_RS21465	4304116..4304406	+	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
ECW_RS21470	4305011..4305229	+	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
ECW_RS21475	4305448..4305690	+	243	WP_001086388.1	hypothetical protein	-
ECW_RS21485	4306020..4306949	-	930	WP_000027702.1	formate dehydrogenase accessory protein FdhE	-
ECW_RS21490	4306946..4307581	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
ECW_RS21495	4307578..4308480	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T27382 WP_000897305.1 NC_017635:4303804-4304115 [Escherichia coli W]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T27382 NC_017635:4303804-4304115 [Escherichia coli W]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT27382 WP_000356397.1 NC_017635:4304116-4304406 [Escherichia coli W]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT27382 NC_017635:4304116-4304406 [Escherichia coli W]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7