Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 3681287..3682014 Replicon chromosome
Accession NZ_CP119120
Organism Escherichia coli strain GENOMIC22-004

Toxin (Protein)


Gene name higB Uniprot ID J7Q991
Locus tag PYZ88_RS17900 Protein ID WP_000547564.1
Coordinates 3681703..3682014 (-) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag PYZ88_RS17895 Protein ID WP_000126294.1
Coordinates 3681287..3681706 (-) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PYZ88_RS17875 (3676337) 3676337..3676864 - 528 WP_001078777.1 electron transport protein HydN -
PYZ88_RS17880 (3677013) 3677013..3678023 - 1011 WP_001392554.1 DNA-binding transcriptional regulator AscG -
PYZ88_RS17885 (3678283) 3678283..3679740 + 1458 WP_001107861.1 PTS cellobiose/arbutin/salicin transporter subunit IIBC -
PYZ88_RS17890 (3679749) 3679749..3681173 + 1425 WP_000110363.1 6-phospho-beta-glucosidase AscB -
PYZ88_RS17895 (3681287) 3681287..3681706 - 420 WP_000126294.1 helix-turn-helix domain-containing protein Antitoxin
PYZ88_RS17900 (3681703) 3681703..3682014 - 312 WP_000547564.1 type II toxin-antitoxin system HigB family toxin Toxin
PYZ88_RS17905 (3682188) 3682188..3682949 - 762 WP_001026446.1 hypothetical protein -
PYZ88_RS17910 (3682974) 3682974..3683444 - 471 WP_000132961.1 hydrogenase maturation peptidase HycI -
PYZ88_RS17915 (3683437) 3683437..3683847 - 411 WP_001291921.1 formate hydrogenlyase assembly protein HycH -
PYZ88_RS17920 (3683844) 3683844..3684611 - 768 WP_000067399.1 formate hydrogenlyase subunit HycG -
PYZ88_RS17925 (3684611) 3684611..3685153 - 543 WP_000493792.1 formate hydrogenlyase subunit HycF -
PYZ88_RS17930 (3685163) 3685163..3686872 - 1710 WP_001288134.1 formate hydrogenlyase subunit HycE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(22-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(85-138)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12426.13 Da        Isoelectric Point: 9.7248

>T273791 WP_000547564.1 NZ_CP119120:c3682014-3681703 [Escherichia coli]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFRYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Download         Length: 312 bp

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15416.42 Da        Isoelectric Point: 4.4596

>AT273791 WP_000126294.1 NZ_CP119120:c3681706-3681287 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Download         Length: 420 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A0FJG5

Antitoxin

Source ID Structure

References