27375

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2227818..2228320 Replicon chromosome
Accession NC_017635
Organism Escherichia coli W

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag ECW_RS11195 Protein ID WP_000767829.1
Coordinates 2227818..2228072 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag ECW_RS11200 Protein ID WP_001259255.1
Coordinates 2228069..2228320 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECW_RS11170 2222833..2223060 - 228 WP_000985273.1 sulfurtransferase TusA family protein -
ECW_RS11175 2223074..2224132 - 1059 WP_000492346.1 transport protein YeeE -
ECW_RS11180 2224311..2225669 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
ECW_RS27610 2225659..2225721 - 63 WP_010723108.1 membrane protein YoeI -
ECW_RS11185 2225936..2226865 - 930 WP_000803351.1 LysR family transcriptional regulator -
ECW_RS11190 2226911..2227735 - 825 WP_000754749.1 SDR family oxidoreductase -
ECW_RS11195 2227818..2228072 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
ECW_RS11200 2228069..2228320 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
ECW_RS26005 2228603..2228653 + 51 WP_001364200.1 his operon leader peptide -
ECW_RS11205 2228799..2229698 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
ECW_RS11210 2229704..2231008 + 1305 WP_001349967.1 histidinol dehydrogenase -
ECW_RS11215 2231005..2232075 + 1071 WP_000108957.1 histidinol-phosphate transaminase -
ECW_RS11220 2232075..2233142 + 1068 WP_000080093.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T27375 WP_000767829.1 NC_017635:c2228072-2227818 [Escherichia coli W]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T27375 NC_017635:c2228072-2227818 [Escherichia coli W]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT27375 WP_001259255.1 NC_017635:c2228320-2228069 [Escherichia coli W]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT27375 NC_017635:c2228320-2228069 [Escherichia coli W]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References