27375

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2227818..2228320 Replicon chromosome
Accession NC_017635
Organism Escherichia coli W

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag ECW_RS11195 Protein ID WP_000767829.1
Coordinates 2227818..2228072 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag ECW_RS11200 Protein ID WP_001259255.1
Coordinates 2228069..2228320 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECW_RS11170 2222833..2223060 - 228 WP_000985273.1 sulfurtransferase TusA family protein -
ECW_RS11175 2223074..2224132 - 1059 WP_000492346.1 transport protein YeeE -
ECW_RS11180 2224311..2225669 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
ECW_RS27610 2225659..2225721 - 63 WP_010723108.1 membrane protein YoeI -
ECW_RS11185 2225936..2226865 - 930 WP_000803351.1 LysR family transcriptional regulator -
ECW_RS11190 2226911..2227735 - 825 WP_000754749.1 SDR family oxidoreductase -
ECW_RS11195 2227818..2228072 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
ECW_RS11200 2228069..2228320 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
ECW_RS26005 2228603..2228653 + 51 WP_001364200.1 his operon leader peptide -
ECW_RS11205 2228799..2229698 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
ECW_RS11210 2229704..2231008 + 1305 WP_001349967.1 histidinol dehydrogenase -
ECW_RS11215 2231005..2232075 + 1071 WP_000108957.1 histidinol-phosphate transaminase -
ECW_RS11220 2232075..2233142 + 1068 WP_000080093.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T27375 WP_000767829.1 NC_017635:c2228072-2227818 [Escherichia coli W]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T27375 NC_017635:c2228072-2227818 [Escherichia coli W]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT27375 WP_001259255.1 NC_017635:c2228320-2228069 [Escherichia coli W]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT27375 NC_017635:c2228320-2228069 [Escherichia coli W]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LNR0

References