Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 52966..53618 Replicon plasmid pK520-TEM
Accession NZ_CP118992
Organism Aeromonas allosaccharophila strain K520

Toxin (Protein)


Gene name higB Uniprot ID A0A6I4WW70
Locus tag PYU98_RS25810 Protein ID WP_024943279.1
Coordinates 52966..53316 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag PYU98_RS25815 Protein ID WP_024943280.1
Coordinates 53316..53618 (+) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PYU98_RS25775 (PYU98_25775) 48381..49331 - 951 Protein_51 IS4 family transposase -
PYU98_RS25780 (PYU98_25780) 49330..49977 + 648 WP_270808117.1 IS5 family transposase -
PYU98_RS25785 (PYU98_25785) 49971..50327 - 357 WP_270808119.1 hypothetical protein -
PYU98_RS25790 (PYU98_25790) 50504..51205 + 702 WP_182928087.1 gamma-glutamyl-gamma-aminobutyrate hydrolase family protein -
PYU98_RS25795 (PYU98_25795) 51372..51569 + 198 WP_275058444.1 hypothetical protein -
PYU98_RS25800 (PYU98_25800) 51604..52521 + 918 WP_270808123.1 IS5 family transposase -
PYU98_RS25805 (PYU98_25805) 52662..52907 + 246 WP_024943278.1 hypothetical protein -
PYU98_RS25810 (PYU98_25810) 52966..53316 + 351 WP_024943279.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
PYU98_RS25815 (PYU98_25815) 53316..53618 + 303 WP_024943280.1 helix-turn-helix transcriptional regulator Antitoxin
PYU98_RS25820 (PYU98_25820) 53711..54115 - 405 WP_270808127.1 IS200/IS605 family transposase -
PYU98_RS25825 (PYU98_25825) 54172..54849 + 678 WP_275058445.1 transposase -
PYU98_RS25830 (PYU98_25830) 54872..57904 - 3033 WP_039272567.1 Tn3 family transposase -
PYU98_RS25835 (PYU98_25835) 57901..58494 - 594 WP_039272634.1 recombinase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid blaTEM-1C - 1..73881 73881
- inside IScluster/Tn blaTEM-1C - 33161..71358 38197


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(13-106)

Antitoxin

Cro/C1-type helix-turn-helix domain

(32-81)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12937.79 Da        Isoelectric Point: 5.7350

>T273703 WP_024943279.1 NZ_CP118992:52966-53316 [Aeromonas allosaccharophila]
MWTVKTTSLFDVWFDEQDEATQEKVLAGLLALAQGGPSVGRPLVDTIKGSRFTNLKELRVQHQGDPLRAFFAFDPLRQAI
VLCAGNKGGNDKRFYKEMLPVAEAEYAKHLTELEVG

Download         Length: 351 bp

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11137.84 Da        Isoelectric Point: 5.1667

>AT273703 WP_024943280.1 NZ_CP118992:53316-53618 [Aeromonas allosaccharophila]
MGRTLNEMLASRSVDSQQRIQEMANELLLEVRLQALREELEVSQVELASALGISQPAVVAMEQRGNDIKLSTMKRYVEAM
GGKLRVDVELPTGRHVGFNV

Download         Length: 303 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6I4WW70

Antitoxin

Source ID Structure

References