Detailed information of TA system TA027360

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralRA/-
• Location: 1502095..1502466
• Replicon: chromosome
• Accession: NC_017635
• Organism: Escherichia coli W

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: F4VC37
• Locus tag: ECW_RS07435
• Protein ID: WP_001317028.1
• Coordinates: 1502272..1502466 (-)
• Length: 65 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	1502095..1502273 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECW_RS07410	1498050..1499423	+	1374	WP_000123740.1	ATP-dependent RNA helicase DbpA	-
ECW_RS07415	1499552..1500487	-	936	WP_001157422.1	tRNA 2-thiocytidine(32) synthetase TtcA	-
ECW_RS25755	1500539..1501774	-	1236	WP_000040852.1	site-specific integrase	-
ECW_RS07425	1501776..1501991	-	216	WP_000079604.1	excisionase XisR	-
ECW_RS07430	1502070..1502279	-	210	WP_000276809.1	double-strand break reduction protein RcbA	-
-	1502095..1502273	+	179	NuclAT_0	-	Antitoxin
-	1502095..1502273	+	179	NuclAT_0	-	Antitoxin
-	1502095..1502273	+	179	NuclAT_0	-	Antitoxin
-	1502095..1502273	+	179	NuclAT_0	-	Antitoxin
ECW_RS07435	1502272..1502466	-	195	WP_001317028.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
ECW_RS07440	1502523..1503332	-	810	WP_000166319.1	recombination protein RecT	-
ECW_RS07445	1503325..1505925	-	2601	WP_000105152.1	exodeoxyribonuclease VIII	-
ECW_RS07450	1506027..1506302	-	276	WP_001349884.1	hypothetical protein	-
ECW_RS07455	1506377..1506547	-	171	WP_001349883.1	hypothetical protein	-
ECW_RS07460	1506547..1506768	-	222	WP_000560220.1	killing protein KilR	-
ECW_RS25125	1507189..1507341	-	153	WP_001169153.1	DUF1391 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1501776..1517853	16077

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-54)

Sequences

Toxin

Protein

• Length: 65 a.a.
• Molecular weight: 7018.89 Da
• Isoelectric Point: 8.9538

>T27360 WP_001317028.1 NC_017635:c1502466-1502272 [Escherichia coli W]
MRYDNVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALERWNKRTTGNNNGGVHV

Nucleotide

• Length: 195 bp

>T27360 NC_017635:c1502466-1502272 [Escherichia coli W]
ATGAGATATGACAATGTTAAACCATGTCCATTTTGTGGTTGTCCATCAGTAACGGTGAAAGCCATTTCAGGGTATTACCG
AGCGAAGTGTAACGGATGCGAATCCCGAACCGGTTATGGTGGAAGTGAAAAAGAAGCACTCGAAAGATGGAATAAACGAA
CCACTGGAAATAATAATGGAGGTGTTCATGTATAA

Antitoxin

Nucleotide

• Length: 179 bp

>AT27360 NC_017635:1502095-1502273 [Escherichia coli W]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAATTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Structures

Toxin

• AlphaFold DB: A0A7L6LHV7

Antitoxin