Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 276995..277536 Replicon chromosome
Accession NC_017635
Organism Escherichia coli W

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag ECW_RS01260 Protein ID WP_000615976.1
Coordinates 276995..277273 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag ECW_RS01265 Protein ID WP_000729704.1
Coordinates 277276..277536 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECW_RS01245 274577..275155 + 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -
ECW_RS01250 275361..276128 + 768 WP_000333380.1 class II glutamine amidotransferase -
ECW_RS01255 276099..276839 - 741 WP_001225679.1 murein L,D-transpeptidase -
ECW_RS01260 276995..277273 - 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
ECW_RS01265 277276..277536 - 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
ECW_RS01270 277722..278495 + 774 WP_000543895.1 C40 family peptidase -
ECW_RS01275 278552..278908 - 357 WP_001030483.1 type II toxin-antitoxin system HicB family antitoxin -
ECW_RS01280 278901..279179 - 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
ECW_RS01285 279284..281377 - 2094 WP_001140808.1 flagellar biosynthesis protein FlhA -
ECW_RS01290 281361..282500 - 1140 WP_000785662.1 flagellar type III secretion system protein FlhB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - gmhA/lpcA 268754..279179 10425


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T27357 WP_000615976.1 NC_017635:c277273-276995 [Escherichia coli W]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T27357 NC_017635:c277273-276995 [Escherichia coli W]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATGCTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT27357 WP_000729704.1 NC_017635:c277536-277276 [Escherichia coli W]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT27357 NC_017635:c277536-277276 [Escherichia coli W]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References