Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | higBA (relBE)/HigB-HigA |
| Location | 62132..62859 | Replicon | plasmid pCRKP-1 |
| Accession | NZ_CP118902 | ||
| Organism | Klebsiella pneumoniae strain CRKP | ||
Toxin (Protein)
| Gene name | higB | Uniprot ID | A0A7W3D9W1 |
| Locus tag | PXG74_RS26250 | Protein ID | WP_011251285.1 |
| Coordinates | 62548..62859 (-) | Length | 104 a.a. |
Antitoxin (Protein)
| Gene name | higA | Uniprot ID | - |
| Locus tag | PXG74_RS26245 | Protein ID | WP_011251286.1 |
| Coordinates | 62132..62551 (-) | Length | 140 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| PXG74_RS26220 (PXG74_26220) | 57725..59092 | + | 1368 | WP_017900870.1 | formimidoylglutamate deiminase | - |
| PXG74_RS26225 (PXG74_26225) | 59617..60152 | + | 536 | Protein_60 | transposase | - |
| PXG74_RS26230 (PXG74_26230) | 60266..60532 | - | 267 | WP_017900868.1 | hypothetical protein | - |
| PXG74_RS26235 (PXG74_26235) | 60636..61603 | + | 968 | Protein_62 | IS5-like element IS903B family transposase | - |
| PXG74_RS26240 (PXG74_26240) | 61649..62062 | - | 414 | WP_017901337.1 | hypothetical protein | - |
| PXG74_RS26245 (PXG74_26245) | 62132..62551 | - | 420 | WP_011251286.1 | helix-turn-helix domain-containing protein | Antitoxin |
| PXG74_RS26250 (PXG74_26250) | 62548..62859 | - | 312 | WP_011251285.1 | type II toxin-antitoxin system HigB family toxin | Toxin |
| PXG74_RS26255 (PXG74_26255) | 63064..63501 | - | 438 | Protein_66 | DDE-type integrase/transposase/recombinase | - |
| PXG74_RS26260 (PXG74_26260) | 63615..63992 | + | 378 | WP_004118218.1 | transposase | - |
| PXG74_RS26265 (PXG74_26265) | 63989..64336 | + | 348 | WP_032430752.1 | IS66 family insertion sequence element accessory protein TnpB | - |
| PXG74_RS26270 (PXG74_26270) | 64385..65923 | + | 1539 | WP_017901237.1 | IS66 family transposase | - |
| PXG74_RS26275 (PXG74_26275) | 65963..66142 | + | 180 | Protein_70 | IS5/IS1182 family transposase | - |
| PXG74_RS26280 (PXG74_26280) | 66688..67668 | - | 981 | WP_012569499.1 | IS5-like element ISKpn26 family transposase | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Non-Mobilizable plasmid | tet(B) / ARR-3 / dfrA27 / aadA16 / qacE / sul1 / qnrB4 / blaDHA-1 | pla | 1..262673 | 262673 | |
| - | inside | IScluster/Tn | - | - | 59934..67668 | 7734 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 104 a.a. Molecular weight: 12346.12 Da Isoelectric Point: 9.7248
>T273545 WP_011251285.1 NZ_CP118902:c62859-62548 [Klebsiella pneumoniae]
MHVISKEPFEEAAKRYPNDSLAIRALYRLVRETDFSSPAEMLTLIPSLDNFKYRNKWWVLDVGGNNLRVIAYINFVNKRF
YVKHIATHADYDKLTRYYRENKE
MHVISKEPFEEAAKRYPNDSLAIRALYRLVRETDFSSPAEMLTLIPSLDNFKYRNKWWVLDVGGNNLRVIAYINFVNKRF
YVKHIATHADYDKLTRYYRENKE
Download Length: 312 bp
Antitoxin
Download Length: 140 a.a. Molecular weight: 15377.46 Da Isoelectric Point: 4.4420
>AT273545 WP_011251286.1 NZ_CP118902:c62551-62132 [Klebsiella pneumoniae]
MITDTAKAIEATKQLVAAVPFLGGSSSESDYREAMELVDYLIENDDENPLIDFLASKIADYEDNSPRFAEFNKAIAEIPV
GVALLRTLIDQHKLSYSDLKDEIGSKSLVSQILSGQRSLTITHIKALSARFGVKPEWFL
MITDTAKAIEATKQLVAAVPFLGGSSSESDYREAMELVDYLIENDDENPLIDFLASKIADYEDNSPRFAEFNKAIAEIPV
GVALLRTLIDQHKLSYSDLKDEIGSKSLVSQILSGQRSLTITHIKALSARFGVKPEWFL
Download Length: 420 bp
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|