Detailed information of TA system TA027353

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): symER/SymE(toxin)
• Location: 4681978..4682390
• Replicon: chromosome
• Accession: NC_017634
• Organism: Escherichia coli O83:H1 str. NRG 857C

Toxin (Protein)

• Gene name: symE
• Uniprot ID: S1NWQ7
• Locus tag: NRG857_RS22450
• Protein ID: WP_000132614.1
• Coordinates: 4681978..4682319 (-)
• Length: 114 a.a.

Antitoxin (RNA)

Gene name	symR
Locus tag	-
Coordinates	4682314..4682390 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
NRG857_RS22430	4677047..4678327	-	1281	WP_014640274.1	DUF445 domain-containing protein	-
NRG857_RS22435	4678512..4679432	+	921	WP_000181192.1	Rpn family recombination-promoting nuclease/putative transposase	-
NRG857_RS22440	4679660..4679740	+	81	WP_020233658.1	hypothetical protein	-
NRG857_RS22445	4679838..4681931	+	2094	WP_000648245.1	DUF262 domain-containing protein	-
NRG857_RS22450	4681978..4682319	-	342	WP_000132614.1	endoribonuclease SymE	Toxin
-	4682314..4682390	+	77	NuclAT_6	-	Antitoxin
-	4682314..4682390	+	77	NuclAT_6	-	Antitoxin
-	4682314..4682390	+	77	NuclAT_6	-	Antitoxin
-	4682314..4682390	+	77	NuclAT_6	-	Antitoxin
-	4682314..4682390	+	77	NuclAT_7	-	Antitoxin
-	4682314..4682390	+	77	NuclAT_7	-	Antitoxin
-	4682314..4682390	+	77	NuclAT_7	-	Antitoxin
-	4682314..4682390	+	77	NuclAT_7	-	Antitoxin
NRG857_RS22455	4682540..4684246	-	1707	WP_001100193.1	restriction endonuclease subunit S	-
NRG857_RS22460	4684246..4685715	-	1470	WP_001540814.1	type I restriction-modification system subunit M	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SymE-like (20-71)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12266.07 Da
• Isoelectric Point: 8.4982

>T27353 WP_000132614.1 NC_017634:c4682319-4681978 [Escherichia coli O83:H1 str. NRG 857C]
MTDTHSIAQPFEAEVSPANNRQLTVSYASRYPDYSRIPAITLKGQWLEAAGFATGTAVDVKVMEGCIVLTAQPPAAAESE
LMQSLRQVCKLSARKQRQVQEFIGVIAGKQKVA

Nucleotide

• Length: 342 bp

>T27353 NC_017634:c4682319-4681978 [Escherichia coli O83:H1 str. NRG 857C]
ATGACTGACACGCATTCTATTGCACAACCGTTCGAAGCAGAAGTCTCCCCGGCAAATAACCGTCAATTAACCGTGAGTTA
TGCGAGTCGCTACCCGGATTACAGCCGTATTCCCGCCATCACCCTGAAAGGTCAGTGGCTGGAAGCCGCCGGTTTTGCCA
CCGGCACTGCGGTAGATGTCAAAGTAATGGAAGGCTGTATTGTCCTCACCGCCCAACCACCCGCCGCCGCCGAGAGCGAA
CTGATGCAGTCGCTGCGCCAGGTGTGCAAGCTGTCGGCGCGTAAACAAAGGCAGGTGCAGGAGTTTATTGGGGTGATTGC
GGGTAAACAGAAAGTCGCCTGA

Antitoxin

Nucleotide

• Length: 77 bp

>AT27353 NC_017634:4682314-4682390 [Escherichia coli O83:H1 str. NRG 857C]
AGTCATAACTGCTATTCCCTATAAATAGTGATTGTGATTAGCGGTGCGGGTGTGTTGGCGCACATCCGCACCGCGCT

Structures

Toxin

• AlphaFold DB: S1NWQ7

Antitoxin