Detailed information of TA system TA027351

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4121686..4122288
• Replicon: chromosome
• Accession: NC_017634
• Organism: Escherichia coli O83:H1 str. NRG 857C

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7U9FTF1
• Locus tag: NRG857_RS19860
• Protein ID: WP_000897301.1
• Coordinates: 4121686..4121997 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: NRG857_RS19865
• Protein ID: WP_000356397.1
• Coordinates: 4121998..4122288 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
NRG857_RS19835	4117601..4118200	+	600	WP_001314324.1	glucose-1-phosphatase	-
NRG857_RS19840	4118194..4119066	+	873	WP_000920762.1	virulence factor BrkB family protein	-
NRG857_RS19845	4119063..4119500	+	438	WP_000560978.1	D-tyrosyl-tRNA(Tyr) deacylase	-
NRG857_RS19850	4119545..4120486	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
NRG857_RS19855	4120550..4121458	-	909	WP_001331553.1	alpha/beta hydrolase	-
NRG857_RS19860	4121686..4121997	+	312	WP_000897301.1	hypothetical protein	Toxin
NRG857_RS19865	4121998..4122288	+	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
NRG857_RS19870	4122647..4122925	+	279	WP_001296612.1	hypothetical protein	-
NRG857_RS19875	4123321..4123539	+	219	WP_001314326.1	ribbon-helix-helix domain-containing protein	-
NRG857_RS19880	4123755..4124684	-	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
NRG857_RS19885	4124681..4125316	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
NRG857_RS19890	4125313..4126215	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12150.14 Da
• Isoelectric Point: 8.8941

>T27351 WP_000897301.1 NC_017634:4121686-4121997 [Escherichia coli O83:H1 str. NRG 857C]
MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKCAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T27351 NC_017634:4121686-4121997 [Escherichia coli O83:H1 str. NRG 857C]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGAACGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTCTCAGGAG
GAAAGGGGAAATGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT27351 WP_000356397.1 NC_017634:4121998-4122288 [Escherichia coli O83:H1 str. NRG 857C]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT27351 NC_017634:4121998-4122288 [Escherichia coli O83:H1 str. NRG 857C]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A7U9FTF1