Detailed information of TA system TA027348

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 3298738..3299537
• Replicon: chromosome
• Accession: NC_017634
• Organism: Escherichia coli O83:H1 str. NRG 857C

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: Q8FDB4
• Locus tag: NRG857_RS15890
• Protein ID: WP_000347252.1
• Coordinates: 3299073..3299537 (+)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: S1PPV5
• Locus tag: NRG857_RS15885
• Protein ID: WP_001296435.1
• Coordinates: 3298738..3299073 (+)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
NRG857_RS15870	3294523..3295293	-	771	WP_001058227.1	2-dehydro-3-deoxyglucarate aldolase	-
NRG857_RS15875	3295309..3296643	-	1335	WP_000979025.1	galactarate/glucarate/glycerate transporter GarP	-
NRG857_RS15880	3297018..3298589	+	1572	WP_001273939.1	galactarate dehydratase	-
NRG857_RS15885	3298738..3299073	+	336	WP_001296435.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
NRG857_RS15890	3299073..3299537	+	465	WP_000347252.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
NRG857_RS15895	3299592..3300401	-	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
NRG857_RS15900	3300650..3301930	+	1281	WP_000681923.1	tagatose-bisphosphate aldolase subunit KbaZ	-
NRG857_RS15905	3301953..3302426	+	474	WP_001298322.1	PTS N-acetylgalactosamine transporter subunit IIB	-
NRG857_RS15910	3302437..3303216	+	780	WP_000406214.1	PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC	-
NRG857_RS15915	3303206..3304084	+	879	WP_001298314.1	PTS system mannose/fructose/sorbose family transporter subunit IID	-
NRG857_RS15920	3304102..3304536	+	435	WP_000948824.1	PTS sugar transporter subunit IIA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17750.11 Da
• Isoelectric Point: 9.4947

>T27348 WP_000347252.1 NC_017634:3299073-3299537 [Escherichia coli O83:H1 str. NRG 857C]
MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEESH

Nucleotide

• Length: 465 bp

>T27348 NC_017634:3299073-3299537 [Escherichia coli O83:H1 str. NRG 857C]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGATGCTTTAGTTGC
CGAAGTCGAGGCATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCTGCCACAAAGTTATTGGCGGTGGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGCTATCGTCTCTTCTTTCGTTACAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACGCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGTAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAAGCCTCACCCAAGAGACAGAAGAAAGCCATTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12321.92 Da
• Isoelectric Point: 4.5392

>AT27348 WP_001296435.1 NC_017634:3298738-3299073 [Escherichia coli O83:H1 str. NRG 857C]
MPANARSNAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFDIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT27348 NC_017634:3298738-3299073 [Escherichia coli O83:H1 str. NRG 857C]
ATGCCCGCTAATGCTCGCTCTAACGCTGTACTGACCACTGAATCAAAGGTCACTATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCTGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCGACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGACGATGAGAT
TGGCGACGACGAATAA

Structures

Toxin

• AlphaFold DB: Q8FDB4

Antitoxin

• AlphaFold DB: S1PPV5