Detailed information of TA system TA027345

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-YefM
• Location: 2117235..2117737
• Replicon: chromosome
• Accession: NC_017634
• Organism: Escherichia coli O83:H1 str. NRG 857C

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: E2QNP2
• Locus tag: NRG857_RS10510
• Protein ID: WP_000767819.1
• Coordinates: 2117235..2117489 (-)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: E2QNP3
• Locus tag: NRG857_RS10515
• Protein ID: WP_001259253.1
• Coordinates: 2117486..2117737 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
NRG857_RS10490	2113728..2115086	-	1359	WP_000019194.1	putrescine/proton symporter PlaP	-
NRG857_RS10495	2115076..2115138	-	63	WP_010723108.1	membrane protein YoeI	-
NRG857_RS10500	2115353..2116282	-	930	WP_000803366.1	LysR family transcriptional regulator	-
NRG857_RS10505	2116328..2117152	-	825	WP_000754733.1	SDR family oxidoreductase	-
NRG857_RS10510	2117235..2117489	-	255	WP_000767819.1	type II toxin-antitoxin system mRNA interferase toxin YoeB	Toxin
NRG857_RS10515	2117486..2117737	-	252	WP_001259253.1	YoeB-YefM toxin-antitoxin system antitoxin YefM	Antitoxin
NRG857_RS10520	2118020..2118070	+	51	WP_001368802.1	his operon leader peptide	-
NRG857_RS10525	2118216..2119115	+	900	WP_000131791.1	ATP phosphoribosyltransferase	-
NRG857_RS10530	2119121..2120425	+	1305	WP_000009596.1	histidinol dehydrogenase	-
NRG857_RS10535	2120422..2121492	+	1071	WP_000108931.1	histidinol-phosphate transaminase	-
NRG857_RS10540	2121492..2122559	+	1068	WP_000080093.1	bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (2-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-72)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 10110.53 Da
• Isoelectric Point: 7.2749

>T27345 WP_000767819.1 NC_017634:c2117489-2117235 [Escherichia coli O83:H1 str. NRG 857C]
VKLIWSEESWDDYLYWQEADKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHLLVYAVTDDSLLIAAC
RYHY

Nucleotide

• Length: 255 bp

>T27345 NC_017634:c2117489-2117235 [Escherichia coli O83:H1 str. NRG 857C]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAGCAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAACTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCTTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9365.62 Da
• Isoelectric Point: 4.6127

>AT27345 WP_001259253.1 NC_017634:c2117737-2117486 [Escherichia coli O83:H1 str. NRG 857C]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSDKGTEKD
IIE

Nucleotide

• Length: 252 bp

>AT27345 NC_017634:c2117737-2117486 [Escherichia coli O83:H1 str. NRG 857C]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGACAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Structures

Toxin

• AlphaFold DB: E2QNP2

Antitoxin

• AlphaFold DB: E2QNP3