Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 1526229..1526791 Replicon chromosome
Accession NC_017634
Organism Escherichia coli O83:H1 str. NRG 857C

Toxin (Protein)


Gene name higB Uniprot ID S1Q1V8
Locus tag NRG857_RS07485 Protein ID WP_000605675.1
Coordinates 1526513..1526791 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag NRG857_RS07480 Protein ID WP_000781370.1
Coordinates 1526229..1526513 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NRG857_RS07465 1521410..1524457 + 3048 WP_015912497.1 formate dehydrogenase-N subunit alpha -
NRG857_RS07470 1524470..1525354 + 885 WP_001240581.1 formate dehydrogenase N subunit beta -
NRG857_RS07475 1525347..1526000 + 654 WP_000045647.1 formate dehydrogenase-N subunit gamma -
NRG857_RS07480 1526229..1526513 - 285 WP_000781370.1 HigA family addiction module antidote protein Antitoxin
NRG857_RS07485 1526513..1526791 - 279 WP_000605675.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
NRG857_RS07490 1526977..1527987 - 1011 WP_000642412.1 alcohol dehydrogenase AdhP -
NRG857_RS07495 1528121..1529818 - 1698 WP_000433464.1 malate dehydrogenase -
NRG857_RS07500 1529975..1530112 - 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
NRG857_RS07505 1530214..1530429 - 216 WP_000495766.1 biofilm-dependent modulation protein -
NRG857_RS07510 1530774..1531205 + 432 WP_000152305.1 peroxiredoxin OsmC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10537.16 Da        Isoelectric Point: 7.3206

>T27334 WP_000605675.1 NC_017634:c1526791-1526513 [Escherichia coli O83:H1 str. NRG 857C]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAACCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

>T27334 NC_017634:c1526791-1526513 [Escherichia coli O83:H1 str. NRG 857C]
ATGATAATGAATTTCAGACACAAGGGATTGCGTGACTTGTTTCTTCTCGGCAAAACCTCCGGCGTTATACCGACGCAAGT
CAAACGATTACGCCACCGACTCGCTGTGATTGATGCAGCATGCTGTCTTGCTGATATCGATATGCCCGGTTACCGACTAC
ATCCGTTAAGCGGCGATCGCGATGGAATTTGGGCGATATCTGTCTCGGGCAACTGGCGAATCACATTTGAATTCGTCAAT
GGCGATGCATACATACTGGATTACGAGGACTATCACTGA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT27334 WP_000781370.1 NC_017634:c1526513-1526229 [Escherichia coli O83:H1 str. NRG 857C]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp

>AT27334 NC_017634:c1526513-1526229 [Escherichia coli O83:H1 str. NRG 857C]
ATGAAAATGGCCAATCATCCCCGCCCGGGGGACATTATTCAGGAATCACTGGACGAACTTAATGTCAGCCTGCGCGAGTT
TGCCAGAGCAATGGAAATTGCGCCCTCAACGGCAAGCCGATTGCTGACCGGAAAAGCAGCTTTGACGCCAGAAATGGCAA
TAAAACTGTCTGTGGTGATCGGCAGTTCGCCGCAAATGTGGCTGAATCTGCAAAATGCCTGGAGTCTGGCAGAGGCAGAA
AAAACAGTGGATGTGTCGCGACTGCGCCGTCTGGTTACGCAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1Q1V8

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References