Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 613296..614095 Replicon chromosome
Accession NZ_CP118601
Organism Escherichia coli strain NB4833

Toxin (Protein)


Gene name yhaV Uniprot ID U9XVR9
Locus tag OU688_RS03005 Protein ID WP_000347267.1
Coordinates 613296..613760 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag OU688_RS03010 Protein ID WP_001307405.1
Coordinates 613760..614095 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OU688_RS02975 (608297) 608297..608731 - 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
OU688_RS02980 (608749) 608749..609627 - 879 WP_001380079.1 PTS N-acetylgalactosamine transporter subunit IID -
OU688_RS02985 (609617) 609617..610396 - 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
OU688_RS02990 (610407) 610407..610880 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
OU688_RS02995 (610903) 610903..612183 - 1281 WP_000681920.1 tagatose-bisphosphate aldolase subunit KbaZ -
OU688_RS03000 (612432) 612432..613241 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
OU688_RS03005 (613296) 613296..613760 - 465 WP_000347267.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
OU688_RS03010 (613760) 613760..614095 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
OU688_RS03015 (614244) 614244..615815 - 1572 WP_001273741.1 galactarate dehydratase -
OU688_RS03020 (616190) 616190..617524 + 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
OU688_RS03025 (617540) 617540..618310 + 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 613296..624751 11455


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17807.17 Da        Isoelectric Point: 9.4947

>T273269 WP_000347267.1 NZ_CP118601:c613760-613296 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT273269 WP_001307405.1 NZ_CP118601:c614095-613760 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XTR4

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References