Detailed information of TA system TA273180

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 457265..457832
• Replicon: chromosome
• Accession: NZ_CP118539
• Organism: Xylella fastidiosa strain ICMP 15197

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q87EE4
• Locus tag: PXH81_RS01895
• Protein ID: WP_004090695.1
• Coordinates: 457265..457546 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: graA
• Uniprot ID: -
• Locus tag: PXH81_RS01900
• Protein ID: WP_004085172.1
• Coordinates: 457557..457832 (+)
• Length: 92 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PXH81_RS01870 (PXH81_01870)	453688..454377	-	690	WP_012382453.1	contractile injection system protein, VgrG/Pvc8 family	-
PXH81_RS01875 (PXH81_01875)	454307..455278	-	972	WP_011097609.1	hypothetical protein	-
PXH81_RS01880 (PXH81_01880)	455286..455843	-	558	WP_011097610.1	phage tail protein I	-
PXH81_RS01885 (PXH81_01885)	455836..456729	-	894	WP_274265311.1	baseplate J/gp47 family protein	-
PXH81_RS01890 (PXH81_01890)	456729..457091	-	363	WP_014607615.1	GPW/gp25 family protein	-
PXH81_RS01895 (PXH81_01895)	457265..457546	+	282	WP_004090695.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
PXH81_RS01900 (PXH81_01900)	457557..457832	+	276	WP_004085172.1	HigA family addiction module antitoxin	Antitoxin
PXH81_RS01905 (PXH81_01905)	457920..458222	+	303	WP_004090696.1	type II toxin-antitoxin system MqsR family toxin	-
PXH81_RS01910 (PXH81_01910)	458225..458626	+	402	WP_004090697.1	type II toxin-antitoxin system MqsA family antitoxin	-
PXH81_RS01915 (PXH81_01915)	458695..459282	-	588	WP_011097612.1	phage baseplate assembly protein V	-
PXH81_RS01920 (PXH81_01920)	459279..459821	-	543	WP_011097613.1	hypothetical protein	-
PXH81_RS01925 (PXH81_01925)	459797..460318	-	522	WP_011097614.1	hypothetical protein	-
PXH81_RS01930 (PXH81_01930)	460315..460638	-	324	WP_011097935.1	hypothetical protein	-
PXH81_RS01935 (PXH81_01935)	460638..460898	-	261	WP_011097616.1	hypothetical protein	-
PXH81_RS01940 (PXH81_01940)	460916..462790	-	1875	WP_038232803.1	phage major capsid protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	445863..469776	23913

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (3-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (15-64)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10639.21 Da
• Isoelectric Point: 10.0980

>T273180 WP_004090695.1 NZ_CP118539:457265-457546 [Xylella fastidiosa]
MIKSFRHKGIQQFFLKGSTAGIQTKHAAKLRIQLTALESAKRPEDMNAPGWKLHPLKGADLKGHWSIWVNGNYRLTFAFE
GEDAILVDYQDYH

Nucleotide

• Length: 282 bp

>T273180 NZ_CP118539:457265-457546 [Xylella fastidiosa]
ATGATCAAAAGTTTCCGCCACAAAGGCATTCAACAATTCTTCCTCAAGGGATCAACCGCTGGTATCCAAACCAAACACGC
CGCCAAACTGCGTATCCAGCTCACCGCGTTGGAGAGTGCCAAGCGCCCCGAAGATATGAATGCTCCAGGCTGGAAACTGC
ACCCACTAAAAGGCGCTGACTTAAAAGGTCATTGGTCCATCTGGGTGAATGGCAACTATCGCCTCACGTTTGCTTTTGAA
GGCGAAGACGCAATCTTGGTCGACTATCAGGATTATCACTAG

Antitoxin

Protein

• Length: 92 a.a.
• Molecular weight: 10072.68 Da
• Isoelectric Point: 10.2184

>AT273180 WP_004085172.1 NZ_CP118539:457557-457832 [Xylella fastidiosa]
MKRMHNPAHPGEVLREYLGELTVTNAAAKLGVSRVALSRILNGTNGISADMALRLEDALGTSAEMWAAMQFKYDLWVASQ
QPRPKITRLHV

Nucleotide

• Length: 276 bp

>AT273180 NZ_CP118539:457557-457832 [Xylella fastidiosa]
ATGAAACGTATGCACAATCCCGCACATCCAGGTGAAGTGTTGCGTGAGTATCTTGGTGAGCTCACTGTGACCAATGCGGC
TGCAAAGCTAGGCGTCAGCCGTGTGGCTTTGTCGCGTATTCTCAATGGTACCAATGGCATCTCTGCCGATATGGCCCTTC
GCCTGGAAGATGCGCTTGGCACCAGTGCAGAGATGTGGGCAGCCATGCAGTTCAAGTATGACCTGTGGGTTGCTAGTCAG
CAGCCACGCCCGAAGATCACGCGCCTTCATGTTTAG

Structures

Toxin

• AlphaFold DB: Q87EE4