Detailed information of TA system TA027298

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4100363..4100965
• Replicon: chromosome
• Accession: NC_017632
• Organism: Escherichia coli UM146

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S1P416
• Locus tag: UM146_RS20415
• Protein ID: WP_000897302.1
• Coordinates: 4100363..4100674 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: UM146_RS20420
• Protein ID: WP_000356397.1
• Coordinates: 4100675..4100965 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
UM146_RS20390	4096277..4096876	+	600	WP_001314324.1	glucose-1-phosphatase	-
UM146_RS20395	4096870..4097742	+	873	WP_000920762.1	virulence factor BrkB family protein	-
UM146_RS20400	4097739..4098176	+	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
UM146_RS20405	4098221..4099162	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
UM146_RS20410	4099226..4100134	-	909	WP_001331553.1	alpha/beta hydrolase	-
UM146_RS20415	4100363..4100674	+	312	WP_000897302.1	hypothetical protein	Toxin
UM146_RS20420	4100675..4100965	+	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
UM146_RS20430	4101324..4101602	+	279	WP_001296612.1	hypothetical protein	-
UM146_RS20435	4101997..4102215	+	219	WP_001314326.1	ribbon-helix-helix domain-containing protein	-
UM146_RS20440	4102431..4103360	-	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
UM146_RS20445	4103357..4103992	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
UM146_RS20450	4103989..4104891	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12203.19 Da
• Isoelectric Point: 9.7791

>T27298 WP_000897302.1 NC_017632:4100363-4100674 [Escherichia coli UM146]
MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T27298 NC_017632:4100363-4100674 [Escherichia coli UM146]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGAACGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTCTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT27298 WP_000356397.1 NC_017632:4100675-4100965 [Escherichia coli UM146]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT27298 NC_017632:4100675-4100965 [Escherichia coli UM146]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: S1P416