Detailed information of TA system TA027297

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3332439..3333133
• Replicon: chromosome
• Accession: NC_017632
• Organism: Escherichia coli UM146

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1PJQ2
• Locus tag: UM146_RS16635
• Protein ID: WP_001263500.1
• Coordinates: 3332439..3332837 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: UM146_RS16640
• Protein ID: WP_000554758.1
• Coordinates: 3332840..3333133 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
UM146_RS16610	3327804..3328262	-	459	WP_001291988.1	xanthine phosphoribosyltransferase	-
UM146_RS16615	3328523..3329980	+	1458	WP_001292999.1	cytosol nonspecific dipeptidase	-
UM146_RS16620	3330037..3330651	-	615	WP_000602123.1	peptide chain release factor H	-
UM146_RS16625	3330648..3331787	-	1140	WP_000521577.1	RNA ligase RtcB family protein	-
UM146_RS16630	3331977..3332429	-	453	WP_001059895.1	GNAT family N-acetyltransferase	-
UM146_RS16635	3332439..3332837	-	399	WP_001263500.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
UM146_RS16640	3332840..3333133	-	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
UM146_RS16645	3333185..3334240	-	1056	WP_001226168.1	DNA polymerase IV	-
UM146_RS16650	3334311..3335096	-	786	WP_000207544.1	putative lateral flagellar export/assembly protein LafU	-
UM146_RS16655	3335068..3336780	+	1713	Protein_3155	flagellar biosynthesis protein FlhA	-
UM146_RS28755	3336859..3337104	+	246	WP_128888917.1	hypothetical protein	-
UM146_RS16660	3337099..3337596	-	498	WP_000006241.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	gmhA/lpcA	3332439..3352792	20353

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15413.85 Da
• Isoelectric Point: 8.9511

>T27297 WP_001263500.1 NC_017632:c3332837-3332439 [Escherichia coli UM146]
MRVFKTKLIRLQLTAEELEALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDAAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T27297 NC_017632:c3332837-3332439 [Escherichia coli UM146]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGAAGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGTGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGCAGCGCATTTGGTATATTGTCAGGGCGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT27297 WP_000554758.1 NC_017632:c3333133-3332840 [Escherichia coli UM146]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT27297 NC_017632:c3333133-3332840 [Escherichia coli UM146]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
TGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTTGAAGCATTAATGGACATGCTTG
CAGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCGAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: S1PJQ2

Antitoxin

• AlphaFold DB: S1QAE3