Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HTH_XRE
Location 3090827..3091506 Replicon chromosome
Accession NC_017632
Organism Escherichia coli UM146

Toxin (Protein)


Gene name higB Uniprot ID V0VBP6
Locus tag UM146_RS15510 Protein ID WP_000057524.1
Coordinates 3091204..3091506 (-) Length 101 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1QAY3
Locus tag UM146_RS15505 Protein ID WP_000806442.1
Coordinates 3090827..3091168 (-) Length 114 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
UM146_RS15495 3087071..3088003 - 933 WP_000883052.1 glutaminase A -
UM146_RS15500 3088265..3090769 + 2505 WP_000083945.1 copper-exporting P-type ATPase CopA -
UM146_RS15505 3090827..3091168 - 342 WP_000806442.1 HigA family addiction module antidote protein Antitoxin
UM146_RS15510 3091204..3091506 - 303 WP_000057524.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
UM146_RS15515 3091639..3092433 + 795 WP_000365177.1 TraB/GumN family protein -
UM146_RS15520 3092637..3093115 + 479 Protein_2934 Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK -
UM146_RS15525 3093139..3093939 + 801 WP_000439796.1 hypothetical protein -
UM146_RS15530 3093936..3094439 + 504 WP_000667000.1 hypothetical protein -
UM146_RS15535 3094477..3096129 - 1653 WP_000771762.1 bifunctional UDP-sugar hydrolase/5'-nucleotidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 3083516..3094439 10923


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(9-98)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-100)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11839.47 Da        Isoelectric Point: 10.2237

>T27295 WP_000057524.1 NC_017632:c3091506-3091204 [Escherichia coli UM146]
MAQKKNIRSFRDAWLADFFVHSTPHRKIPAEIHTTLSRKLDIINAATSHWDLRSPPGNRYEELSGKLQEYSSIRVNKQYR
LIFKWVNGKAEELFLDPHNY

Download         Length: 303 bp

>T27295 NC_017632:c3091506-3091204 [Escherichia coli UM146]
ATGGCTCAAAAGAAAAATATAAGGAGTTTTCGTGATGCCTGGTTGGCTGATTTTTTTGTCCATTCAACGCCACATCGAAA
GATCCCTGCTGAAATCCATACCACGTTATCACGAAAGTTGGACATCATTAATGCTGCCACTTCTCATTGGGATTTACGCT
CCCCTCCGGGGAACCGATACGAAGAGTTATCCGGGAAATTACAGGAATACTCCTCCATTAGAGTGAATAAACAGTACCGA
CTGATTTTTAAATGGGTTAATGGCAAGGCCGAAGAATTGTTTTTAGACCCACACAACTACTAA

Antitoxin


Download         Length: 114 a.a.        Molecular weight: 13171.10 Da        Isoelectric Point: 5.7790

>AT27295 WP_000806442.1 NC_017632:c3091168-3090827 [Escherichia coli UM146]
MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSALINNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLW
EVENNMRTQEELGRIETVAEYLARREERAKKVA

Download         Length: 342 bp

>AT27295 NC_017632:c3091168-3090827 [Escherichia coli UM146]
ATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAAT
CAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCACTGATCAATAACAATCGTAAACTCACCACTG
AGATGGCATTCCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCAGTTGATCTTTGG
GAAGTTGAAAACAACATGCGTACCCAGGAAGAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGA
GCGTGCAAAAAAGGTCGCGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0VBP6

Antitoxin

Source ID Structure
PDB 2EBY
AlphaFold DB S1QAY3

References