Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2024425..2024987 Replicon chromosome
Accession NC_017632
Organism Escherichia coli UM146

Toxin (Protein)


Gene name higB Uniprot ID S1Q1V8
Locus tag UM146_RS09935 Protein ID WP_000605675.1
Coordinates 2024425..2024703 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag UM146_RS09940 Protein ID WP_000781370.1
Coordinates 2024703..2024987 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
UM146_RS09910 2020012..2020443 - 432 WP_000152310.1 peroxiredoxin OsmC -
UM146_RS09915 2020788..2021003 + 216 WP_000495766.1 biofilm-dependent modulation protein -
UM146_RS09920 2021105..2021242 + 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
UM146_RS09925 2021398..2023095 + 1698 WP_000433462.1 malate dehydrogenase -
UM146_RS09930 2023229..2024239 + 1011 WP_000642412.1 alcohol dehydrogenase AdhP -
UM146_RS09935 2024425..2024703 + 279 WP_000605675.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
UM146_RS09940 2024703..2024987 + 285 WP_000781370.1 HigA family addiction module antidote protein Antitoxin
UM146_RS09945 2025216..2025869 - 654 WP_000045647.1 formate dehydrogenase-N subunit gamma -
UM146_RS09950 2025862..2026746 - 885 WP_001240584.1 formate dehydrogenase N subunit beta -
UM146_RS09955 2026759..2029806 - 3048 WP_011478186.1 formate dehydrogenase-N subunit alpha -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10537.16 Da        Isoelectric Point: 7.3206

>T27293 WP_000605675.1 NC_017632:2024425-2024703 [Escherichia coli UM146]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAACCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

>T27293 NC_017632:2024425-2024703 [Escherichia coli UM146]
ATGATAATGAATTTCAGACACAAGGGATTGCGTGACTTGTTTCTTCTCGGCAAAACCTCCGGCGTTATACCGACGCAAGT
CAAACGATTACGCCACCGACTCGCTGTGATTGATGCAGCATGCTGTCTTGCTGATATCGATATGCCCGGTTACCGACTAC
ATCCGTTAAGCGGCGATCGCGATGGAATTTGGGCGATATCTGTCTCGGGCAACTGGCGAATCACATTTGAATTCGTCAAT
GGCGATGCATACATACTGGATTACGAGGACTATCACTGA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT27293 WP_000781370.1 NC_017632:2024703-2024987 [Escherichia coli UM146]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp

>AT27293 NC_017632:2024703-2024987 [Escherichia coli UM146]
ATGAAAATGGCCAATCATCCCCGCCCGGGGGACATTATTCAGGAATCACTGGACGAACTTAATGTCAGCCTGCGCGAGTT
TGCCAGAGCAATGGAAATTGCGCCCTCAACGGCAAGCCGATTGCTGACCGGAAAAGCAGCTTTGACGCCAGAAATGGCAA
TAAAACTTTCCGTGGTGATCGGCAGTTCGCCGCAAATGTGGCTGAATCTGCAAAATGCCTGGAGTCTGGCAGAGGCGGAA
AAAACGGTGGATGTGTCGCGACTGCGCCGTCTGGTTACGCAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1Q1V8

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References