Detailed information of TA system TA027274

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4470578..4471180
• Replicon: chromosome
• Accession: NC_017631
• Organism: Escherichia coli ABU 83972

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S1P416
• Locus tag: ECABU_RS22155
• Protein ID: WP_000897302.1
• Coordinates: 4470578..4470889 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: ECABU_RS22160
• Protein ID: WP_000356397.1
• Coordinates: 4470890..4471180 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECABU_RS22130	4466491..4467090	+	600	WP_001443191.1	glucose-1-phosphatase	-
ECABU_RS22135	4467084..4467956	+	873	WP_000920762.1	virulence factor BrkB family protein	-
ECABU_RS22140	4467953..4468390	+	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
ECABU_RS22145	4468435..4469376	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
ECABU_RS22150	4469440..4470348	-	909	WP_001305059.1	alpha/beta hydrolase	-
ECABU_RS22155	4470578..4470889	+	312	WP_000897302.1	hypothetical protein	Toxin
ECABU_RS22160	4470890..4471180	+	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
ECABU_RS22170	4471539..4471817	+	279	WP_001296612.1	hypothetical protein	-
ECABU_RS22175	4472213..4472431	+	219	WP_001314326.1	ribbon-helix-helix domain-containing protein	-
ECABU_RS22180	4472647..4473576	-	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
ECABU_RS22185	4473573..4474208	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
ECABU_RS22190	4474205..4475107	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12203.19 Da
• Isoelectric Point: 9.7791

>T27274 WP_000897302.1 NC_017631:4470578-4470889 [Escherichia coli ABU 83972]
MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T27274 NC_017631:4470578-4470889 [Escherichia coli ABU 83972]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGAACGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTCTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT27274 WP_000356397.1 NC_017631:4470890-4471180 [Escherichia coli ABU 83972]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT27274 NC_017631:4470890-4471180 [Escherichia coli ABU 83972]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: S1P416