Detailed information of TA system TA027272

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 3618463..3619262
• Replicon: chromosome
• Accession: NC_017631
• Organism: Escherichia coli ABU 83972

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: Q8FDB4
• Locus tag: ECABU_RS17875
• Protein ID: WP_000347252.1
• Coordinates: 3618798..3619262 (+)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: S1PPV5
• Locus tag: ECABU_RS17870
• Protein ID: WP_001296435.1
• Coordinates: 3618463..3618798 (+)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECABU_RS17855	3614248..3615018	-	771	WP_001058223.1	2-dehydro-3-deoxyglucarate aldolase	-
ECABU_RS17860	3615034..3616368	-	1335	WP_000979025.1	galactarate/glucarate/glycerate transporter GarP	-
ECABU_RS17865	3616743..3618314	+	1572	WP_001273940.1	galactarate dehydratase	-
ECABU_RS17870	3618463..3618798	+	336	WP_001296435.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
ECABU_RS17875	3618798..3619262	+	465	WP_000347252.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
ECABU_RS17880	3619317..3620126	-	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
ECABU_RS17885	3620375..3621655	+	1281	WP_000681925.1	tagatose-bisphosphate aldolase subunit KbaZ	-
ECABU_RS17890	3621678..3622151	+	474	WP_001295547.1	PTS N-acetylgalactosamine transporter subunit IIB	-
ECABU_RS17895	3622162..3622941	+	780	WP_000406214.1	PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC	-
ECABU_RS17900	3622931..3623809	+	879	WP_001298314.1	PTS system mannose/fructose/sorbose family transporter subunit IID	-
ECABU_RS17905	3623827..3624261	+	435	WP_000948824.1	PTS sugar transporter subunit IIA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17750.11 Da
• Isoelectric Point: 9.4947

>T27272 WP_000347252.1 NC_017631:3618798-3619262 [Escherichia coli ABU 83972]
MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEESH

Nucleotide

• Length: 465 bp

>T27272 NC_017631:3618798-3619262 [Escherichia coli ABU 83972]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGATGCTTTAGTTGC
CGAAGTCGAGGCATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCTGCCACAAAGTTATTGGCGGTGGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGCGCTGGTCGCTATCGTCTCTTCTTTCGTTACAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACGCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGTAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAAGCCTCACCCAAGAGACAGAAGAAAGCCATTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12321.92 Da
• Isoelectric Point: 4.5392

>AT27272 WP_001296435.1 NC_017631:3618463-3618798 [Escherichia coli ABU 83972]
MPANARSNAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFDIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT27272 NC_017631:3618463-3618798 [Escherichia coli ABU 83972]
ATGCCCGCTAATGCTCGCTCTAACGCTGTACTGACCACTGAATCAAAGGTCACTATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCTGGCCTGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCGACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGACGATGAGAT
TGGCGACGACGAATAA

Structures

Toxin

• AlphaFold DB: Q8FDB4

Antitoxin

• AlphaFold DB: S1PPV5