Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 4526793..4527334 Replicon chromosome
Accession NZ_CP118014
Organism Escherichia coli strain B-766

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag PT280_RS22265 Protein ID WP_000615976.1
Coordinates 4527056..4527334 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag PT280_RS22260 Protein ID WP_000729704.1
Coordinates 4526793..4527053 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PT280_RS22235 (4521829) 4521829..4522968 + 1140 WP_000785662.1 flagellar type III secretion system protein FlhB -
PT280_RS22240 (4522952) 4522952..4525045 + 2094 WP_001140808.1 flagellar biosynthesis protein FlhA -
PT280_RS22245 (4525150) 4525150..4525428 + 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
PT280_RS22250 (4525421) 4525421..4525777 + 357 WP_001030483.1 type II toxin-antitoxin system HicB family antitoxin -
PT280_RS22255 (4525834) 4525834..4526607 - 774 WP_000543895.1 C40 family peptidase -
PT280_RS22260 (4526793) 4526793..4527053 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
PT280_RS22265 (4527056) 4527056..4527334 + 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
PT280_RS22270 (4527490) 4527490..4528230 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
PT280_RS22275 (4528201) 4528201..4528968 - 768 WP_000333380.1 class II glutamine amidotransferase -
PT280_RS22280 (4529174) 4529174..4529752 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - gmhA/lpcA 4525421..4536362 10941


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T272688 WP_000615976.1 NZ_CP118014:4527056-4527334 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT272688 WP_000729704.1 NZ_CP118014:4526793-4527053 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References