Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2349694..2350196 Replicon chromosome
Accession NC_017631
Organism Escherichia coli ABU 83972

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag ECABU_RS11905 Protein ID WP_000767829.1
Coordinates 2349694..2349948 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag ECABU_RS11910 Protein ID WP_001259255.1
Coordinates 2349945..2350196 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECABU_RS11890 2346187..2347545 - 1359 WP_000019194.1 putrescine/proton symporter PlaP -
ECABU_RS28635 2347535..2347597 - 63 WP_010723108.1 membrane protein YoeI -
ECABU_RS11895 2347812..2348741 - 930 WP_000803366.1 LysR family transcriptional regulator -
ECABU_RS11900 2348787..2349611 - 825 WP_000754737.1 SDR family oxidoreductase -
ECABU_RS11905 2349694..2349948 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
ECABU_RS11910 2349945..2350196 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
ECABU_RS26640 2350479..2350529 + 51 WP_001364200.1 his operon leader peptide -
ECABU_RS11915 2350675..2351574 + 900 WP_000131767.1 ATP phosphoribosyltransferase -
ECABU_RS11920 2351580..2352884 + 1305 WP_001529283.1 histidinol dehydrogenase -
ECABU_RS11925 2352881..2353951 + 1071 WP_000108948.1 histidinol-phosphate transaminase -
ECABU_RS11930 2353951..2355018 + 1068 WP_000080128.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T27268 WP_000767829.1 NC_017631:c2349948-2349694 [Escherichia coli ABU 83972]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T27268 NC_017631:c2349948-2349694 [Escherichia coli ABU 83972]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT27268 WP_001259255.1 NC_017631:c2350196-2349945 [Escherichia coli ABU 83972]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT27268 NC_017631:c2350196-2349945 [Escherichia coli ABU 83972]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References