Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 4122943..4123484 Replicon chromosome
Accession NZ_CP117971
Organism Escherichia coli strain B-1109

Toxin (Protein)


Gene name yafQ Uniprot ID Q47149
Locus tag PT723_RS20345 Protein ID WP_000615983.1
Coordinates 4123206..4123484 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID Q47150
Locus tag PT723_RS20340 Protein ID WP_000729703.1
Coordinates 4122943..4123203 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PT723_RS20320 (4118618) 4118618..4119403 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
PT723_RS20325 (4119375) 4119375..4121087 + 1713 Protein_3962 flagellar biosynthesis protein FlhA -
PT723_RS20330 (4121311) 4121311..4121808 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
PT723_RS20335 (4121984) 4121984..4122733 - 750 WP_000056849.1 C40 family peptidase -
PT723_RS20340 (4122943) 4122943..4123203 + 261 WP_000729703.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
PT723_RS20345 (4123206) 4123206..4123484 + 279 WP_000615983.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
PT723_RS20350 (4123640) 4123640..4124380 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
PT723_RS20355 (4124351) 4124351..4125118 - 768 WP_000333380.1 class II glutamine amidotransferase -
PT723_RS20360 (4125223) 4125223..4125801 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - gmhA/lpcA / tssA / hcp1/tssD1 4116746..4142719 25973


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(4-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-85)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10846.62 Da        Isoelectric Point: 9.9941

>T272606 WP_000615983.1 NZ_CP117971:4123206-4123484 [Escherichia coli]
MIQRDIEYSGQYSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9405.80 Da        Isoelectric Point: 5.0758

>AT272606 WP_000729703.1 NZ_CP117971:4122943-4123203 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGIDVHKAKDADDL
FDKLGI

Download         Length: 261 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4Q2U

Antitoxin

Source ID Structure
PDB 4Q2U
AlphaFold DB A0A5F1F716

References