Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 997119..997846 Replicon chromosome
Accession NZ_CP117971
Organism Escherichia coli strain B-1109

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag PT723_RS04825 Protein ID WP_000550189.1
Coordinates 997119..997433 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag PT723_RS04830 Protein ID WP_000560266.1
Coordinates 997430..997846 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PT723_RS04805 (993286) 993286..994272 - 987 WP_001301393.1 Gfo/Idh/MocA family oxidoreductase -
PT723_RS04810 (994351) 994351..995034 - 684 WP_001183046.1 vancomycin high temperature exclusion protein -
PT723_RS04815 (995111) 995111..995614 - 504 WP_001295542.1 M48 family metallopeptidase -
PT723_RS04820 (995699) 995699..996835 + 1137 WP_000018695.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
PT723_RS04825 (997119) 997119..997433 + 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
PT723_RS04830 (997430) 997430..997846 + 417 WP_000560266.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
PT723_RS04835 (997891) 997891..999909 - 2019 WP_137044542.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
PT723_RS04840 (1000335) 1000335..1002686 - 2352 WP_137044544.1 alpha-glucosidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T272591 WP_000550189.1 NZ_CP117971:997119-997433 [Escherichia coli]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 14995.42 Da        Isoelectric Point: 4.4547

>AT272591 WP_000560266.1 NZ_CP117971:997430-997846 [Escherichia coli]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References