Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 1707888..1708450 Replicon chromosome
Accession NC_017631
Organism Escherichia coli ABU 83972

Toxin (Protein)


Gene name higB Uniprot ID S1Q1V8
Locus tag ECABU_RS08700 Protein ID WP_000605675.1
Coordinates 1708172..1708450 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag ECABU_RS08695 Protein ID WP_000781370.1
Coordinates 1707888..1708172 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECABU_RS08680 1703069..1706116 + 3048 WP_011076399.1 formate dehydrogenase-N subunit alpha -
ECABU_RS08685 1706129..1707013 + 885 WP_001240584.1 formate dehydrogenase N subunit beta -
ECABU_RS08690 1707006..1707659 + 654 WP_000045646.1 formate dehydrogenase-N subunit gamma -
ECABU_RS08695 1707888..1708172 - 285 WP_000781370.1 HigA family addiction module antidote protein Antitoxin
ECABU_RS08700 1708172..1708450 - 279 WP_000605675.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
ECABU_RS08705 1708636..1709646 - 1011 WP_000642412.1 alcohol dehydrogenase AdhP -
ECABU_RS08710 1709780..1711477 - 1698 WP_000433460.1 malate dehydrogenase -
ECABU_RS08715 1711634..1711771 - 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
ECABU_RS08720 1711873..1712088 - 216 WP_000495766.1 biofilm-dependent modulation protein -
ECABU_RS08725 1712433..1712864 + 432 WP_000152307.1 peroxiredoxin OsmC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10537.16 Da        Isoelectric Point: 7.3206

>T27257 WP_000605675.1 NC_017631:c1708450-1708172 [Escherichia coli ABU 83972]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAACCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

>T27257 NC_017631:c1708450-1708172 [Escherichia coli ABU 83972]
ATGATAATGAATTTCAGACACAAGGGATTGCGTGACTTGTTTCTTCTCGGCAAAACCTCCGGCGTTATACCGACGCAAGT
CAAACGATTACGCCACCGACTCGCTGTGATTGATGCAGCATGCTGTCTTGCTGATATCGATATGCCCGGTTACCGACTAC
ATCCGTTAAGCGGCGATCGCGATGGAATTTGGGCGATATCTGTCTCGGGTAACTGGCGAATCACATTTGAATTCGTCAAT
GGCGATGCATACATACTGGATTACGAGGACTATCACTGA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT27257 WP_000781370.1 NC_017631:c1708172-1707888 [Escherichia coli ABU 83972]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp

>AT27257 NC_017631:c1708172-1707888 [Escherichia coli ABU 83972]
ATGAAAATGGCCAATCATCCCCGCCCGGGGGACATTATTCAGGAATCACTGGACGAACTTAATGTCAGCCTGCGCGAGTT
TGCCAGAGCAATGGAAATTGCGCCCTCTACGGCAAGTCGATTGCTGACCGGAAAAGCAGCTTTGACGCCAGAAATGGCAA
TAAAACTGTCTGTGGTGATCGGCAGTTCGCCGCAAATGTGGCTGAATCTGCAAAATGCCTGGAGTCTGGCAGAGGCAGAA
AAAACAGTGGATGTGTCGCGACTGCGCCGTCTGGTTACGCAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1Q1V8

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References