Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 1264557..1265098 Replicon chromosome
Accession NZ_CP117962
Organism Escherichia coli strain JM109

Toxin (Protein)


Gene name yafQ Uniprot ID Q47149
Locus tag PT299_RS07450 Protein ID WP_000615983.1
Coordinates 1264820..1265098 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID Q47150
Locus tag PT299_RS07445 Protein ID WP_000729703.1
Coordinates 1264557..1264817 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PT299_RS07420 (1260088) 1260088..1260257 - 170 Protein_1216 hypothetical protein -
PT299_RS07425 (1260502) 1260502..1261515 - 1014 WP_001013499.1 4-hydroxy-2-oxovalerate aldolase -
PT299_RS07430 (1261505) 1261505..1262701 + 1197 Protein_1218 FHIPEP family type III secretion protein -
PT299_RS07435 (1262925) 1262925..1263422 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
PT299_RS07440 (1263598) 1263598..1264347 - 750 WP_000056849.1 C40 family peptidase -
PT299_RS07445 (1264557) 1264557..1264817 + 261 WP_000729703.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
PT299_RS07450 (1264820) 1264820..1265098 + 279 WP_000615983.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
PT299_RS07455 (1265254) 1265254..1265994 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
PT299_RS07460 (1265965) 1265965..1266732 - 768 WP_000333380.1 class II glutamine amidotransferase -
PT299_RS07465 (1266938) 1266938..1267516 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island formA gmhA/lpcA 1250767..1277032 26265


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(4-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-85)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10846.62 Da        Isoelectric Point: 9.9941

>T272545 WP_000615983.1 NZ_CP117962:1264820-1265098 [Escherichia coli]
MIQRDIEYSGQYSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9405.80 Da        Isoelectric Point: 5.0758

>AT272545 WP_000729703.1 NZ_CP117962:1264557-1264817 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGIDVHKAKDADDL
FDKLGI

Download         Length: 261 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4Q2U

Antitoxin

Source ID Structure
PDB 4Q2U
AlphaFold DB A0A5F1F716

References