Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 225993..226534 Replicon plasmid unnamed1
Accession NZ_CP117961
Organism Escherichia coli strain JM109

Toxin (Protein)


Gene name yafQ Uniprot ID Q47149
Locus tag PT299_RS01180 Protein ID WP_000615983.1
Coordinates 225993..226271 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID Q47150
Locus tag PT299_RS01185 Protein ID WP_000729703.1
Coordinates 226274..226534 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PT299_RS01155 (221899) 221899..222068 + 170 Protein_229 hypothetical protein -
PT299_RS01160 (222232) 222232..223443 + 1212 WP_000107627.1 3-(3-hydroxy-phenyl)propionate transporter -
PT299_RS01165 (223545) 223545..224084 + 540 WP_001096705.1 DUF2058 domain-containing protein -
PT299_RS01170 (224359) 224359..225126 + 768 WP_000333380.1 class II glutamine amidotransferase -
PT299_RS01175 (225097) 225097..225837 - 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
PT299_RS01180 (225993) 225993..226271 - 279 WP_000615983.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
PT299_RS01185 (226274) 226274..226534 - 261 WP_000729703.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
PT299_RS01190 (226744) 226744..227493 + 750 WP_000056849.1 C40 family peptidase -
PT299_RS01195 (227669) 227669..228166 + 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
PT299_RS01200 (228390) 228390..230102 - 1713 Protein_238 flagellar biosynthesis protein FlhA -
PT299_RS01205 (230074) 230074..230859 + 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - yagV/ecpE / yagW/ecpD / yagX/ecpC / yagY/ecpB / yagZ/ecpA / ykgK/ecpR / fdeC 1..231544 231544


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(4-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-85)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10846.62 Da        Isoelectric Point: 9.9941

>T272540 WP_000615983.1 NZ_CP117961:c226271-225993 [Escherichia coli]
MIQRDIEYSGQYSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9405.80 Da        Isoelectric Point: 5.0758

>AT272540 WP_000729703.1 NZ_CP117961:c226534-226274 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGIDVHKAKDADDL
FDKLGI

Download         Length: 261 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4Q2U

Antitoxin

Source ID Structure
PDB 4Q2U
AlphaFold DB A0A5F1F716

References