Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 489..1183 Replicon plasmid unnamed1
Accession NZ_CP117961
Organism Escherichia coli strain JM109

Toxin (Protein)


Gene name yafO Uniprot ID Q47157
Locus tag PT299_RS00015 Protein ID WP_001263489.1
Coordinates 785..1183 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag PT299_RS00010 Protein ID WP_000554758.1
Coordinates 489..782 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PT299_RS00010 (489) 489..782 + 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
PT299_RS00015 (785) 785..1183 + 399 WP_001263489.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
PT299_RS00020 (1193) 1193..1645 + 453 WP_001059892.1 GNAT family N-acetyltransferase -
PT299_RS00025 (1963) 1963..2169 + 207 Protein_4 RtcB family protein -
PT299_RS00030 (2165) 2165..2686 + 522 Protein_5 peptide chain release factor H -
PT299_RS00035 (2743) 2743..4200 - 1458 WP_001292994.1 cytosol nonspecific dipeptidase -
PT299_RS00040 (4461) 4461..4919 + 459 WP_001291990.1 xanthine phosphoribosyltransferase -
- (5515) 5515..5595 + 81 NuclAT_1 - -
- (5515) 5515..5595 + 81 NuclAT_1 - -
- (5515) 5515..5595 + 81 NuclAT_1 - -
- (5515) 5515..5595 + 81 NuclAT_1 - -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - yagV/ecpE / yagW/ecpD / yagX/ecpC / yagY/ecpB / yagZ/ecpA / ykgK/ecpR / fdeC 1..231544 231544


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15487.84 Da        Isoelectric Point: 7.4215

>T272533 WP_001263489.1 NZ_CP117961:785-1183 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT272533 WP_000554758.1 NZ_CP117961:489-782 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090J8B1

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References