Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 3326513..3327240 Replicon chromosome
Accession NZ_CP117960
Organism Escherichia coli strain B-3008

Toxin (Protein)


Gene name higB Uniprot ID S1EX98
Locus tag PT288_RS16155 Protein ID WP_000550192.1
Coordinates 3326513..3326827 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag PT288_RS16160 Protein ID WP_000560266.1
Coordinates 3326824..3327240 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PT288_RS16135 (3322680) 3322680..3323666 - 987 WP_000617698.1 Gfo/Idh/MocA family oxidoreductase -
PT288_RS16140 (3323745) 3323745..3324428 - 684 WP_001183046.1 vancomycin high temperature exclusion protein -
PT288_RS16145 (3324505) 3324505..3325008 - 504 WP_001295542.1 M48 family metallopeptidase -
PT288_RS16150 (3325093) 3325093..3326229 + 1137 WP_000018695.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
PT288_RS16155 (3326513) 3326513..3326827 + 315 WP_000550192.1 type II toxin-antitoxin system toxin HigB Toxin
PT288_RS16160 (3326824) 3326824..3327240 + 417 WP_000560266.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
PT288_RS16165 (3327285) 3327285..3329303 - 2019 WP_000121433.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
PT288_RS16170 (3329729) 3329729..3332080 - 2352 WP_000695486.1 alpha-glucosidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12063.08 Da        Isoelectric Point: 10.0409

>T272525 WP_000550192.1 NZ_CP117960:3326513-3326827 [Escherichia coli]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTPKEYDFFTAVHRTKGKK

Download         Length: 315 bp

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 14995.42 Da        Isoelectric Point: 4.4547

>AT272525 WP_000560266.1 NZ_CP117960:3326824-3327240 [Escherichia coli]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1EX98

Antitoxin

Source ID Structure

References