Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 1630984..1631525 Replicon chromosome
Accession NZ_CP117960
Organism Escherichia coli strain B-3008

Toxin (Protein)


Gene name yafQ Uniprot ID S1FHQ9
Locus tag PT288_RS08105 Protein ID WP_000615979.1
Coordinates 1631247..1631525 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag PT288_RS08100 Protein ID WP_000729704.1
Coordinates 1630984..1631244 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PT288_RS08085 (1627043) 1627043..1629136 + 2094 WP_001140809.1 flagellar biosynthesis protein FlhA -
PT288_RS08090 (1629352) 1629352..1629849 - 498 WP_000006261.1 REP-associated tyrosine transposase RayT -
PT288_RS08095 (1630025) 1630025..1630798 - 774 WP_001353765.1 C40 family peptidase -
PT288_RS08100 (1630984) 1630984..1631244 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
PT288_RS08105 (1631247) 1631247..1631525 + 279 WP_000615979.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
PT288_RS08110 (1631681) 1631681..1632421 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
PT288_RS08115 (1632392) 1632392..1633159 - 768 WP_000333379.1 class II glutamine amidotransferase -
PT288_RS08120 (1633365) 1633365..1633943 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - gmhA/lpcA 1629352..1650029 20677


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10830.62 Da        Isoelectric Point: 10.0702

>T272519 WP_000615979.1 NZ_CP117960:1631247-1631525 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT272519 WP_000729704.1 NZ_CP117960:1630984-1631244 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1FHQ9

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References